Makino T
Microbiol Immunol. 1983;27(9):749-55. doi: 10.1111/j.1348-0421.1983.tb00640.x.
Protoplasts of Staphylococcus aureus 209P and Cowan 1 were induced by treatment with lysostaphin. These protoplasts were sensitive to detergent, a low concentration of sodium chloride and low temperature. Almost all protoplast cells spread on CLYS agar medium (casein hydrolysate, yeast extract, Na-lactate, and NaCl) formed typical L-form colonies. Horse serum (0.25%) and Mg2+ (100 mM) are essential factors for formation of the L-form colonies of 209P. In the case of Cowan 1, Mg2+ was not required. The active factor(s) in horse serum was heat-resistant and protein in nature.
用溶葡萄球菌素处理金黄色葡萄球菌209P和考恩1型菌株的原生质体。这些原生质体对去污剂、低浓度氯化钠和低温敏感。几乎所有接种在CLYS琼脂培养基（酪蛋白水解物、酵母提取物、乳酸钠和氯化钠）上的原生质体细胞都形成了典型的L型菌落。马血清（0.25%）和Mg2 +（100 mM）是209P菌株L型菌落形成的必需因素。对于考恩1型菌株，不需要Mg2 +。马血清中的活性因子耐热且本质上是蛋白质。
