阐明绿原酸控制摩氏摩根菌生长和组胺形成的潜力。

Elucidating the potential of chlorogenic acid for controlling Morganella psychrotolerans growth and histamine formation.

机构信息

Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.

Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya 572018, China.

出版信息

J Appl Microbiol. 2024 Jan 2;135(1). doi: 10.1093/jambio/lxad308.

DOI:10.1093/jambio/lxad308

PMID:38140945

Abstract

AIM

To investigate the inhibitory impact of chlorogenic acid (CGA) on the growth of Morganella psychrotolerans and its ability to form histamine.

METHODS AND RESULTS

The antimicrobial effect of CGA on M. psychrotolerans was evaluated using the minimum inhibitory concentration (MIC) method, revealing an MIC value of 10 mg ml-1. The alkaline phosphatase (AKP) activity, cell membrane potential, and scanning electron microscopy images revealed that CGA treatment disrupted cell structure and cell membrane. Moreover, CGA treatment led to a dose-dependent decrease in crude histidine decarboxylase (HDC) activity and gene expression of histidine decarboxylase (hdc). Molecular docking analysis demonstrated that CGA interacted with HDC through hydrogen bonds. Furthermore, in situ investigation confirmed the efficacy of CGA in controlling the growth of M. psychrotolerans and significantly reducing histamine formation in raw tuna.

CONCLUSION

CGA had good activity in controlling the growth of M. psychrotolerans and histamine formation.

摘要

目的

研究绿原酸（CGA）对嗜冷摩根菌生长的抑制作用及其产组胺能力。

方法与结果

采用最低抑菌浓度（MIC）法评价 CGA 对嗜冷摩根菌的抑菌效果，结果显示 MIC 值为 10 mg ml-1。碱性磷酸酶（AKP）活性、细胞膜电位和扫描电子显微镜图像显示，CGA 处理破坏了细胞结构和细胞膜。此外，CGA 处理导致粗组氨酸脱羧酶（HDC）活性和组氨酸脱羧酶（hdc）基因表达呈剂量依赖性下降。分子对接分析表明，CGA 通过氢键与 HDC 相互作用。此外，原位研究证实 CGA 能有效控制嗜冷摩根菌的生长，并显著减少生金枪鱼中组胺的形成。