根癌农杆菌T-DNA基因7在非洲爪蟾卵母细胞中的表达。

Expression of Agrobacterium tumefaciens T-DNA gene 7 in Xenopus laevis oocytes.

作者信息

McPherson J C, Kingsbury R

出版信息

Biochem Biophys Res Commun. 1986 Dec 30;141(3):1242-8. doi: 10.1016/s0006-291x(86)80178-4.

Abstract

The expression of Agrobacterium tumefaciens T-DNA gene 7 was investigated in Xenopus laevis oocytes. Cloned DNA injected into oocytes consisted of T-DNA sequences derived from octopine type Ti plasmid B6-806 and T-DNA attached to plant DNA sequences at the left junction in crown gall tumors. Transcription initiation sites observed in oocytes were similar to those for transcript 7 in crown gall tumors. Quantitative differences in transcription occurred depending on the flanking sequences of the injected clones indicating that sequences upstream of the TATA box of T-DNA gene 7 affect the quantitative expression of this gene in Xenopus oocytes.

摘要

在非洲爪蟾卵母细胞中研究了根癌土壤杆菌T-DNA基因7的表达。注入卵母细胞的克隆DNA由源自章鱼碱型Ti质粒B6 - 806的T-DNA序列以及在冠瘿瘤左边界处与植物DNA序列相连的T-DNA组成。在卵母细胞中观察到的转录起始位点与冠瘿瘤中转录本7的转录起始位点相似。转录的定量差异取决于注入克隆的侧翼序列，这表明T-DNA基因7的TATA框上游序列影响该基因在非洲爪蟾卵母细胞中的定量表达。