McPherson J C
Nucleic Acids Res. 1984 Mar 12;12(5):2317-25. doi: 10.1093/nar/12.5.2317.
Crown gall tumor formation involves integration into the plant genome of DNA sequences (the T-region) of tumor-inducing (Ti) plasmids present in Agrobacterium tumefaciens. The T-DNA of the tumor expresses several gene products. Little is known about the function or regulation of expression of the 0.7kb transcript, which represents a relatively abundant T-DNA transcript in octopine-type tumors. In this report, a detailed structural analysis of the gene encoding the 0.7 kb transcript has been obtained by DNA sequence analysis of T-DNA isolated from A6S/2 tumor line. An indication of the structural characteristics of the protein product is obtained from the predicted amino acid sequence. The sequences flanking the open reading frame show characteristics with other eucaryotic genes. The corresponding DNA sequence of the inducing Ti plasmid (pTiA6) is identical with that of the DNA sequence from the tumor. Comparison of this gene sequence with the corresponding region of another Ti plasmid (pTiAch5) shows several differences in the 5' flanking sequence, but the nucleotide sequence of the coding region and 3' flanking region are identical.
冠瘿瘤的形成涉及到根癌土壤杆菌中存在的致瘤（Ti）质粒的DNA序列（T区域）整合到植物基因组中。肿瘤的T-DNA表达几种基因产物。对于在章鱼碱型肿瘤中相对丰富的0.7kb转录本的功能或表达调控了解甚少。在本报告中，通过对从A6S/2肿瘤系分离的T-DNA进行DNA序列分析，获得了编码0.7kb转录本的基因的详细结构分析。从预测的氨基酸序列中可以了解蛋白质产物的结构特征。开放阅读框两侧的序列显示出与其他真核基因的特征。诱导性Ti质粒（pTiA6）的相应DNA序列与来自肿瘤的DNA序列相同。将该基因序列与另一个Ti质粒（pTiAch5）的相应区域进行比较，发现在5'侧翼序列中有几个差异，但编码区和3'侧翼区的核苷酸序列是相同的。
