DeLoach J R, Andrews K
Biotechnol Appl Biochem. 1986 Dec;8(6):537-45.
Hypotonic dialysis of human erythrocytes results in porous cell stability for several days. Hypotonic cells stored for 1 week are essentially normal with respect to the preparation of carrier erythrocytes. Afterward, cells begin to irreversibly hemolyze resulting in decreased cell recoveries and decreased encapsulation percentages of two probes, sucrose and inulin. The holes generated by controlled hypotonic dialysis (100 mOsm/kg) are unlike the single rupture hole generated by dialysis to 10-20 mOsm/kg. The minimum pore size of resealed, annealed carrier cells is confirmed to be less than 5.2 A.
人红细胞的低渗透析可使多孔细胞在数天内保持稳定。储存1周的低渗细胞在制备载体红细胞方面基本正常。此后，细胞开始不可逆地溶血，导致细胞回收率降低以及两种探针（蔗糖和菊粉）的包封率降低。通过控制性低渗透析（100 mOsm/kg）产生的孔与透析至10 - 20 mOsm/kg产生的单个破裂孔不同。重新封闭、退火后的载体细胞的最小孔径经证实小于5.2埃。
