Berhe Birhanu Debesay, Sbhatu Desta Berhe, Munawar T Mohammad, Gebreyohannes Gebreselama
Department of Biological and Chemical Engineering, Mekelle Institute of Technology, Mekelle University, PO Box 1632, Mekelle, Ethiopia.
Heliyon. 2023 Dec 3;9(12):e22955. doi: 10.1016/j.heliyon.2023.e22955. eCollection 2023 Dec.
micropropagation study of Reynolds was conducted using offshoots in Murashige and Skoog (MS) media enriched with plant growth regulators (PGRs). Initiation experiment, carried out by culturing sterilized explants in full-strength MS media enriched with 0.0-1.0 mg/L benzyl amino purine (BAP) alone and in combination with 0.10 mg/L indole-3-butyric acid (IBA), resulted in 89 - 100 % healthy and live (i.e., initiated) explants in 9-29 days. Regeneration study, conducted by culturing initiated explants in full-strength MS media supplemented with 0.0-3.0 mg/L BAP alone and in combination with 0.50 mg/L IBA, showed that treatments enriched with 1.0-3.0 mg/L BAP in combination with 0.50 mg/L IBA yielded better shooting responses than the rest of the treatments. The rooting responses of the shoots were also tested by culturing in half-strength MS media enriched with 0.0-4.0 mg/L NAA alone and in combination with 0.25 mg/L IBA. Better rooting responses were observed in treatments supplemented with 1.0-4.0 mg/L NAA in combination with 0.25 mg/L IBA with two exceptions. The responses of plantlets to primary and secondary acclimatization in greenhouse, nursery shade, and direct sunlight in coco peat, composted soil, and manured soil were excellent - with survival percentages of 90-98 %. The findings of this empirical research are important for developing refined protocol of micropropagation of this ecologically important but endangered plant.
雷诺兹的微繁殖研究使用了在添加植物生长调节剂（PGR）的Murashige和Skoog（MS）培养基中的侧枝进行。启动实验通过将灭菌外植体培养在添加了0.0 - 1.0毫克/升苄氨基嘌呤（BAP）单独以及与0.10毫克/升吲哚 - 3 - 丁酸（IBA）组合的全强度MS培养基中进行，结果在9 - 29天内有89% - 100%的外植体健康存活（即启动）。再生研究通过将启动的外植体培养在添加了0.0 - 3.0毫克/升BAP单独以及与0.50毫克/升IBA组合的全强度MS培养基中进行，结果表明添加1.0 - 3.0毫克/升BAP与0.50毫克/升IBA组合的处理比其他处理产生了更好的芽诱导反应。芽的生根反应也通过在添加了0.0 - 4.0毫克/升萘乙酸（NAA）单独以及与0.25毫克/升IBA组合的半强度MS培养基中培养进行测试。在添加1.0 - 4.0毫克/升NAA与0.25毫克/升IBA组合的处理中观察到了更好的生根反应，但有两个例外。幼苗在温室、苗圃遮荫处以及可可泥炭、堆肥土和施肥土中的直射阳光下进行初次和二次驯化的反应非常好，存活率为90% - 98%。这项实证研究的结果对于制定这种具有生态重要性但濒危植物的微繁殖精细方案很重要。
