Johansson A, Lundborg M, Skog S, Jarstrand C, Camner P
Br J Ind Med. 1987 Jan;44(1):47-52. doi: 10.1136/oem.44.1.47.
Rabbits were exposed to 0.6 mg/m3 of nickel as NiCl2 for about one month. After exposure, alveolar macrophages were lavaged from the lung and divided into three fractions by elutriation. Laminated structures in the macrophages were related to fraction number so that the fractions with the largest cells contained the highest number of structures. The lysozyme activity decreased in unfractionated as well as in fractionated macrophages from nickel exposed rabbits. The decrease was most pronounced in the fraction with the smallest macrophages and smallest number of laminated structures. Therefore the pronounced decrease in lysozyme activity seen in this and earlier studies is not caused by the increased amount of surfactant material. Increased amount of surfactant is a hallmark of nickel inhalation exposure and the surfactant material is responsible for the morphological and metabolic effects of the macrophages. The decreased lysozyme activity is probably a direct effect of nickel on the macrophages.
将兔子暴露于浓度为0.6毫克/立方米的氯化镍形式的镍中约一个月。暴露后，从肺部冲洗出肺泡巨噬细胞，并通过淘洗将其分为三个部分。巨噬细胞中的层状结构与部分编号相关，因此细胞最大的部分包含的结构数量最多。未分级以及分级后的来自镍暴露兔子的巨噬细胞中的溶菌酶活性均降低。在巨噬细胞最小且层状结构数量最少的部分中，这种降低最为明显。因此，在本研究和早期研究中观察到的溶菌酶活性的明显降低并非由表面活性物质数量的增加所致。表面活性物质数量的增加是吸入镍暴露的一个标志，并且表面活性物质是巨噬细胞形态和代谢效应的原因。溶菌酶活性的降低可能是镍对巨噬细胞的直接作用。
