Lundborg M, Camner P
Environ Res. 1984 Aug;34(2):335-42. doi: 10.1016/0013-9351(84)90102-6.
Groups of rabbits were exposed to chlorides of nickel, cadmium, copper, and cobalt at concentrations ranging from 0.2 to 0.6 mg/m3 (as metal) for 4-6 weeks (5 days/week, 6 hr/day). Activity of lysozyme (muramidase) in lavage fluid, in alveolar macrophages, and in culture medium from macrophages incubated at 37 degrees C for 1 and 20 hr was estimated using the lyso-plate technique, agar plates with heat-killed Micrococcus lysodeikticus. In the nickel-exposed rabbits lysozyme activity in the mucous membrane from the left main bronchus was also estimated. Following nickel exposure the lysozyme level was significantly decreased in lavage fluid, macrophages, and in culture medium from incubated macrophages but remained unchanged in the mucous membrane. After exposure to cadmium, copper, and cobalt, lysozyme levels increased or were unchanged.
将兔分组，使其暴露于浓度范围为0.2至0.6毫克/立方米（以金属计）的镍、镉、铜和钴的氯化物中，持续4至6周（每周5天，每天6小时）。使用溶菌板技术，即含有热灭活的溶壁微球菌的琼脂平板，来估计灌洗液、肺泡巨噬细胞以及在37摄氏度下孵育1小时和20小时的巨噬细胞培养基中的溶菌酶（胞壁质酶）活性。对暴露于镍的兔，还估计了左主支气管黏膜中的溶菌酶活性。镍暴露后，灌洗液、巨噬细胞以及孵育巨噬细胞的培养基中的溶菌酶水平显著降低，但黏膜中的溶菌酶水平保持不变。镉、铜和钴暴露后，溶菌酶水平升高或未改变。
