Osserman E F, Lawlor D P
J Exp Med. 1966 Nov 1;124(5):921-52. doi: 10.1084/jem.124.5.921.
Markedly increased quantities of lysozyme have been found in the serum and urine (ranging to 2.6 g per day) of ten consecutive cases of monocytic and monomyelocytic leukemia. The enzyme has been isolated from the urine of several cases and physicochemically and immunochemically characterized. It is apparently identical to the lysozyme of normal tears, saliva, leukocytes, and serum, but structurally different from the lysozyme of hen's egg white. The activity of the human enzyme assayed with M. lysodeikticus organisms is 3 to 12 times greater than egg white lysozyme at equivalent concentrations. An agar plate method has been developed for quantitating lysozyme activity in small samples (approximately 25 microl) of serum, urine, or other biological fluids. The range and reproducibility of this method were found to be superior to previously available lysozyme assay procedures. Present evidence indicates that lysozyme is the principal, if not the sole, product of the proliferating monocytes in monocytic and monomyelocytic leukemia, and quantitation of serum and urine lysozyme should be a useful diagnostic procedure for these leukemias.
在十例连续的单核细胞性和单核粒细胞性白血病患者的血清和尿液中（每天可达2.6克），已发现溶菌酶的含量显著增加。已从几例患者的尿液中分离出该酶，并对其进行了物理化学和免疫化学特性鉴定。它显然与正常眼泪、唾液、白细胞和血清中的溶菌酶相同，但在结构上与鸡蛋清中的溶菌酶不同。用溶壁微球菌检测时，在等效浓度下，人源酶的活性比鸡蛋清溶菌酶高3至12倍。已开发出一种琼脂平板法，用于定量血清、尿液或其他生物流体的小样本（约25微升）中的溶菌酶活性。发现该方法的范围和重现性优于以前可用的溶菌酶检测程序。目前的证据表明，溶菌酶即使不是单核细胞性和单核粒细胞性白血病中增殖单核细胞的唯一产物，也是主要产物，血清和尿液溶菌酶的定量测定对于这些白血病应是一种有用的诊断方法。
