Lundborg M, Camner P
Toxicology. 1981;22(4):353-8. doi: 10.1016/0300-483x(81)90029-9.
Six rabbits were exposed for 4 months (5 days/week, 6 h/day) and 6 rabbits for 8 months to approx. 0.1 mg/m3 of metallic nickel dust (U.S. threshold limit value (TLV) 1 mg/m3). Another 8 rabbits were exposed for 4-6 weeks (5 days/week, 6 h/day) to 0.3 mg/m3 (as Ni) of nickel chloride (U.S. TLV 0.1 mg/m3). After exposure lungs were lavaged. Concentration of lysozyme in the lavage fluid was estimated with the lyso-plate technique (agar plates with heat-killed Micrococcus lysodeikticus) after macrophages had been removed. All 3 exposed groups had markedly lower concentrations of lysozyme than corresponding controls. Mean values in controls and exposed rabbits were: for 4 months metallic nickel dust exposure 2.3 and less than or equal to 0.04 microgram/ml; for 8 months metallic nickel dust exposure 1.4 and less than or equal to 0.5 microgram/ml; and for nickel chloride exposure 1.9 and less than or equal to 0.4 microgram/ml.
六只兔子暴露于约0.1毫克/立方米的金属镍粉尘中4个月（每周5天，每天6小时），另六只兔子暴露8个月（每周5天，每天6小时），（美国阈限值（TLV）为1毫克/立方米）。另有八只兔子暴露于0.3毫克/立方米（以镍计）的氯化镍中4至6周（每周5天，每天6小时）（美国TLV为0.1毫克/立方米）。暴露结束后对肺部进行灌洗。在去除巨噬细胞后，采用溶菌板技术（含有热灭活溶壁微球菌的琼脂平板）对灌洗液中的溶菌酶浓度进行估算。所有三个暴露组的溶菌酶浓度均明显低于相应的对照组。对照组和暴露组兔子的平均值分别为：暴露于金属镍粉尘4个月的为2.3和小于或等于0.04微克/毫升；暴露于金属镍粉尘8个月的为1.4和小于或等于0.5微克/毫升；暴露于氯化镍的为1.9和小于或等于0.4微克/毫升。
