Chen Wenwen, Zeng Yiwei, Liu Huanying, Sun Dezhi, Liu Xinying, Xu Haiyu, Wu Hongbin, Qiu Bin, Dang Yan
Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-Remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing, China.
Qinglin Chuangneng (Shanghai) Technology Co., Ltd., Shanghai, China.
Front Bioeng Biotechnol. 2023 Dec 11;11:1330293. doi: 10.3389/fbioe.2023.1330293. eCollection 2023.
Garden waste, one type of lignocellulosic biomass, holds significant potential for the production of volatile fatty acids (VFAs) through anaerobic fermentation. However, the hydrolysis efficiency of garden waste is limited by the inherent recalcitrance, which further influences VFA production. Granular activated carbon (GAC) could promote hydrolysis and acidogenesis efficiency during anaerobic fermentation. This study developed a strategy to use GAC to enhance the anaerobic fermentation of garden waste without any complex pretreatments and extra enzymes. The results showed that GAC addition could improve VFA production, especially acetate, and reach the maximum total VFA yield of 191.55 mg/g VS, which increased by 27.35% compared to the control group. The highest VFA/sCOD value of 70.01% was attained in the GAC-amended group, whereas the control group only reached 49.35%, indicating a better hydrolysis and acidogenesis capacity attributed to the addition of GAC. Microbial community results revealed that GAC addition promoted the enrichment of and , which are crucial for anaerobic VFA production. In addition, only the GAC-amended group showed the presence of and genera, which are associated with electron transfer processes. Metagenomics analysis indicated that GAC addition improved the abundance of glycoside hydrolases (GHs) and key functional enzymes related to hydrolysis and acidogenesis. Furthermore, the assessment of major genera influencing functional genes in both groups indicated that , , and were the primary contributors to upregulated genes. These findings underscored the significance of employing GAC to enhance the anaerobic fermentation of garden waste, offering a promising approach for sustainable biomass conversion and VFA production.
园林废弃物作为木质纤维素生物质的一种，通过厌氧发酵生产挥发性脂肪酸（VFAs）具有巨大潜力。然而，园林废弃物的水解效率受到其固有顽固性的限制，这进一步影响了VFAs的生产。颗粒活性炭（GAC）可提高厌氧发酵过程中的水解和产酸效率。本研究开发了一种策略，无需任何复杂预处理和额外酶，利用GAC增强园林废弃物的厌氧发酵。结果表明，添加GAC可提高VFAs产量，尤其是乙酸盐产量，总VFAs产量最高可达191.55 mg/g VS，比对照组提高了27.35%。GAC添加组的VFA/sCOD值最高达到70.01%，而对照组仅为49.35%，这表明添加GAC具有更好的水解和产酸能力。微生物群落结果显示，添加GAC促进了对厌氧VFAs生产至关重要的[具体微生物名称1]和[具体微生物名称2]的富集。此外，只有GAC添加组显示出与电子传递过程相关的[具体微生物名称3]属和[具体微生物名称4]属的存在。宏基因组学分析表明，添加GAC提高了糖苷水解酶（GHs）以及与水解和产酸相关的关键功能酶的丰度。此外，对两组中影响功能基因的主要属的评估表明，[具体属名1]、[具体属名2]和[具体属名3]是基因上调的主要贡献者。这些发现强调了利用GAC增强园林废弃物厌氧发酵的重要性，为可持续生物质转化和VFAs生产提供了一种有前景的方法。
