Kim B S, Massey P B
J Immunol. 1982 Mar;128(3):1145-8.
Mice with the CBA/N defect (xid) are unresponsive to phosphorylcholine (PC), To determine whether idiotype-specific suppressor T cells can also be generated in these defective mice, defective (CBA/N X BALB/c)F1 male and nondefective (CBA/N X BALB/c)F1 female or (BALB/c X CBA/N)F1 male mice were neonatally injected with antibodies specific for the major idiotype of anti-PC antibody, i.e., anti-TEPC-15 idiotype (T15id) antibody. Suppressor cell activity was examined by co-culturing spleen cells from neonatally treated F1 mice with spleen cells of normal nondefective F1 mice in the presence of antigen. Spleen cells from defective (CBA/NM X BALB/c)F1 mice treated with anti-T15id antibody demonstrated a level of suppressor activity (greater than 83% suppression) comparable to that of similarly treated nondefective F1 mice. This suppression was specific for the T15id of anti-PC response, and a Lyt-1-2+-bearing T cell population appeared to be responsible for the active suppression. These suppressor T cells recognized T15 but not PC, based on a functional absorption test. These results indicate that the CBA/N defects, including the deficiency in the anti-PC response by B lymphocytes and a possible T cell defect, do not influence the generation of T15id-specific suppressor T cells by neonatal injection with anti-T15id antibody.
具有CBA/N缺陷（xid）的小鼠对磷酸胆碱（PC）无反应。为了确定在这些缺陷小鼠中是否也能产生独特型特异性抑制性T细胞，对缺陷型（CBA/N×BALB/c）F1雄性小鼠以及非缺陷型（CBA/N×BALB/c）F1雌性小鼠或（BALB/c×CBA/N）F1雄性小鼠在新生期注射针对抗PC抗体主要独特型的抗体，即抗TEPC-15独特型（T15id）抗体。通过在抗原存在的情况下，将新生期处理过的F1小鼠的脾细胞与正常非缺陷F1小鼠的脾细胞共培养来检测抑制细胞活性。用抗T15id抗体处理的缺陷型（CBA/NM×BALB/c）F1小鼠的脾细胞表现出的抑制活性水平（抑制率大于83%）与同样处理的非缺陷F1小鼠相当。这种抑制对抗PC反应的T15id具有特异性，并且一个带有Lyt-1-2 +的T细胞群体似乎是活性抑制的原因。基于功能吸收试验，这些抑制性T细胞识别T15而不是PC。这些结果表明，CBA/N缺陷，包括B淋巴细胞抗PC反应的缺陷以及可能的T细胞缺陷，并不影响通过新生期注射抗T15id抗体产生T15id特异性抑制性T细胞。
