分裂型绿色荧光蛋白标记的核纤层蛋白作为研究LMN-1动力学的工具。

Split-GFP lamin as a tool for studying LMN-1 dynamics .

作者信息

Gregory Ellen F, Ragle James Matthew, Ward Jordan D, Starr Daniel A

机构信息

Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, United States.

Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, California, United States.

出版信息

MicroPubl Biol. 2023 Dec 13;2023. doi: 10.17912/micropub.biology.001022. eCollection 2023.

DOI:10.17912/micropub.biology.001022

PMID:38152058

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10751582/

Abstract

We engineered a fluorescent fusion protein of lamin, by fusing the eleventh beta strand of GFP to the N-terminus of LMN-1 at the endogenous locus. When co-expressed with GFP1-10, GFP11::LMN-1 was observed at the nuclear periphery of a wide variety of somatic cells. Homozygous animals had normal numbers of viable embryos. However, the animals had a mild swimming defect. While not completely functional, the GFP11::LMN-1 strain is more healthy than other published fluorescent LMN-1 lines, making it a valuable reagent for studying lamins.

摘要

我们通过将绿色荧光蛋白（GFP）的第十一个β链融合到内源性位点的LMN-1的N端，构建了一种核纤层蛋白的荧光融合蛋白。当与GFP1-10共表达时，在多种体细胞的核周边观察到GFP11::LMN-1。纯合动物具有正常数量的存活胚胎。然而，这些动物存在轻微的游泳缺陷。虽然GFP11::LMN-1菌株并非完全功能正常，但它比其他已发表的荧光LMN-1品系更健康，使其成为研究核纤层蛋白的有价值试剂。