Parthenais E, Haskill S
J Immunol. 1979 Sep;123(3):1329-33.
The thymus dependency of humoral immunity to syngeneic tumor antigens was investigated in T cell-deficient DBA/2 mice. ATXBM animals bearing the T1699 mammary tumor in the subcutaneous abdominal area displayed normal immediate but not delayed hypersensitivity reactions (DHR) to 3 M KCl-extracted T1699 antigens injected into the footpad. Sera from ATXBM tumor-bearers passively transferred immediate hypersensitivity but failed to support tumor-specific macrophage-mediated ADCC reactions. The synthesis of macrophage-mediated ADCC antibody was greatly reduced in the CXBM animals when compared to nonirradiated tumor-bearers. The CXBM mice, however, showed normal T cell function as measured by allograft rejection, antibody response to sheep erythrocytes, and DHR to T1699 antigens. Of all antibody classes and subclasses tested by indirect membrane fluorescence, only IgG2b was found to be produced at normal levels by either ATXBM or CXBM tumor-bearers. The results show that IgG2b antibody production in response to T1699 syngeneic tumor antigens is thymus independent and suggest that this antibody is the mediator of immediate hypersensitivity. The synthesis of macrophage-mediated ADCC antibody (IgG2a) was found to be not only thymus dependent but also sensitive to the long-term effects of irradiation and bone marrow repopulation.
在T细胞缺陷的DBA/2小鼠中研究了对同基因肿瘤抗原的体液免疫的胸腺依赖性。皮下腹部携带T1699乳腺肿瘤的ATXBM动物对注射到足垫的3M KCl提取的T1699抗原表现出正常的即刻超敏反应，但无迟发型超敏反应（DHR）。ATXBM荷瘤动物的血清被动转移即刻超敏反应，但不能支持肿瘤特异性巨噬细胞介导的ADCC反应。与未照射的荷瘤动物相比，CXBM动物中巨噬细胞介导的ADCC抗体的合成大大减少。然而，通过同种异体移植排斥、对绵羊红细胞的抗体反应以及对T1699抗原的DHR测量，CXBM小鼠显示出正常的T细胞功能。通过间接膜荧光检测的所有抗体类别和亚类中，只有IgG2b在ATXBM或CXBM荷瘤动物中以正常水平产生。结果表明，针对T1699同基因肿瘤抗原的IgG2b抗体产生不依赖胸腺，并表明该抗体是即刻超敏反应的介质。发现巨噬细胞介导的ADCC抗体（IgG2a）的合成不仅依赖胸腺，而且对辐射和骨髓再填充的长期影响敏感。
