College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.
Mikrochim Acta. 2023 Dec 28;191(1):60. doi: 10.1007/s00604-023-06156-5.
A new fluorescence sensing strategy has been developed. Four bimetallic nanoclusters, gold/silver, gold/copper, gold/molybdenum and gold/cobalt, were prepared using bovine serum albumin (BSA) as a reducing and stabilizing agent. The fluorescence properties of four nanoclusters were explored by solid-state UV and XPS. The gold/silver nanoclusters (BSA-Au/Ag NCs) with the best ratiometric fluorescence properties for gallic acid (GA) in plants were selected to realize the sensitive detection of GA. GA affected the conformation of BSA, thereby disrupting the luminescent environment of the nanoclusters, resulting in a pronounced fluorescence quenching at 566 nm. The ratiometric fluorescence signal (I/I) was used for trace detection of GA in plants. It has a wide response range of 1.25-40.0 μM and a low detection limit of 45.27 nM. GA was detected at 19.49 μM in the plant extract, and the spiked recoveries ranged from 96.09 to 104.6%. In addition, due to the non-toxic and biocompatible properties of BSA, BSA-Au/Ag NCs have also been validated for fluorescence imaging of plant tissues. It realized the comparison of GA content in different parts of plants and the difference of GA content in plants after abiotic stress. Therefore, the developed strategy offers potential application for the analytical study of active substances in plants.
一种新的荧光传感策略已经被开发出来。四种双金属纳米簇，金/银、金/铜、金/钼和金/钴，使用牛血清白蛋白（BSA）作为还原剂和稳定剂来制备。通过固态 UV 和 XPS 研究了四种纳米簇的荧光性质。选择具有最佳比率荧光性质的金/银纳米簇（BSA-Au/Ag NCs）用于植物中的没食子酸（GA）的灵敏检测。GA 影响 BSA 的构象，从而破坏纳米簇的发光环境，导致在 566nm 处出现明显的荧光猝灭。比率荧光信号（I/I）用于植物中 GA 的痕量检测。它具有 1.25-40.0 μM 的宽响应范围和 45.27 nM 的低检测限。在植物提取物中检测到 19.49 μM 的 GA，加标回收率在 96.09%至 104.6%之间。此外，由于 BSA 的无毒和生物相容性，BSA-Au/Ag NCs 也已被验证用于植物组织的荧光成像。它实现了植物不同部位 GA 含量的比较以及非生物胁迫后植物中 GA 含量的差异。因此，所开发的策略为植物中活性物质的分析研究提供了潜在的应用。
