College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.
Anal Chem. 2022 Jun 28;94(25):8999-9008. doi: 10.1021/acs.analchem.2c01050. Epub 2022 Jun 15.
Achieving detection of the phytohormone abscisic acid (ABA) is of critical importance for understanding plant growth and development. We report a hybrid supramolecular fluorescent probe that uses bovine serum albumin (BSA) as a host. Aggregation-induced emission of fluorescent chromophores (AIEgens) enables luminescence in the presence of BSA. ABA and its aptamer act as a switch to trigger this fluorescent system, the strategy that exhibits high sensitivity to abscisic acid with a detection limit of 0.098 nM. The probe test strip also enables visualization of ABA content from plants by colorimetric observation with the naked eye. In particular, the high biocompatibility and small molecular size of the prepared fluorescent probe allow for effective monitoring of ABA in plant tissues by fluorescence imaging. This strategy provides a new perspective to achieve the detection of endogenous and exogenous ABA in plants and has important implications for plant biology research.
检测植物激素脱落酸（ABA）对于理解植物生长和发育至关重要。我们报告了一种使用牛血清白蛋白（BSA）作为主体的混合超分子荧光探针。荧光发色团的聚集诱导发射（AIEgens）使得在存在 BSA 的情况下发光。ABA 和它的适体作为一个开关来触发这个荧光系统，该策略对脱落酸表现出高灵敏度，检测限为 0.098 nM。探针测试条还可以通过肉眼比色观察来可视化植物中的 ABA 含量。特别是，所制备的荧光探针的高生物相容性和小分子尺寸允许通过荧光成像有效地监测植物组织中的 ABA。该策略为在植物中实现内源性和外源性 ABA 的检测提供了新的视角，对植物生物学研究具有重要意义。
