Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
School of Fishery, Zhejiang Ocean University, Zhoushan, 316022, China.
J Comp Physiol B. 2020 Jan;190(1):27-34. doi: 10.1007/s00360-019-01247-3. Epub 2019 Nov 25.
Larimichthys polyactis is one of the most economically important marine fish species that have become newly cultured in China in recent years. The gene expression changes that L. polyactis experiences in cold toleranceis still unknown, limiting the expansion of its cultivation, fast growth, and high yield. To investigate the molecular mechanism behind L. polyactis's cold tolerance and to provide a resource for conducting genetic research on L. polyactis, transcriptome sequencing using RNA-seq was performed on individuals that survived cold stress at 4 °C (cold tolerant, CT), and individuals that barely survived 4 °C (cold sensitive, CS), which was considered as the control. A number of 387,607,550 clean reads were obtained from the transcriptomes, and comparative transcriptomic analysis identified 141 differently expressed genes (DEGs), of which 67 were up-regulated and 74 were down-regulated in CT compared to CS under cold stress. Furthermore, ten differently expressed genes were selected from the RNA-Seq analysis to be further validated by real-time PCR. Functional network analysis indicated that L. polyactis adapted to cold stress by employing a series of mechanisms to minimize damages caused by exposure to cold temperatures. The molecular mechanisms identified through RNA-Seq included Extracellular matrix (ECM) receptor interaction, glycerolipid metabolism, regulation of autophagy and focal adhesion pathway as playing vital roles in cold tolerance in L. polyactis. This study may help elucidate how L. polyactis tolerates cold, which is of value for breeding cold-tolerant L. polyactis stocks for cultivation.
黄姑鱼是近年来中国新养殖的最具经济价值的海洋鱼类之一。其在耐寒性方面的基因表达变化尚不清楚，这限制了其养殖范围的扩大、快速生长和高产。为了研究黄姑鱼耐寒性的分子机制，并为黄姑鱼的遗传研究提供资源，我们对在 4°C（耐寒，CT）下幸存的个体和几乎无法在 4°C 下幸存的个体（耐寒，CS）进行了 RNA-seq 转录组测序。从转录组中获得了 387,607,550 条清洁读数，比较转录组分析鉴定出 141 个差异表达基因（DEGs），其中 CT 中 67 个上调，74 个下调。此外，从 RNA-Seq 分析中选择了十个差异表达基因进行实时 PCR 进一步验证。功能网络分析表明，黄姑鱼通过采用一系列机制来最小化暴露于低温造成的损伤来适应冷应激。通过 RNA-Seq 鉴定的分子机制包括细胞外基质 (ECM) 受体相互作用、甘油脂代谢、自噬调控和焦点黏附途径在黄姑鱼耐寒性中起着重要作用。本研究可能有助于阐明黄姑鱼如何耐受寒冷，这对于培育耐寒黄姑鱼种群进行养殖具有重要价值。
