Zhang Xuguang, Zhou Jun, Xu Wengang, Zhan Wei, Zou Huafeng, Lin Jun
Engineering Technology Research Center of Marine Ranching, College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China.
School of Ocean, Yantai University, Yantai 264005, China.
Animals (Basel). 2022 Aug 13;12(16):2061. doi: 10.3390/ani12162061.
Noise has the potential to induce physiological stress in marine fishes, which may lead to all sorts of ecological consequences. In the current study, we used the RNA-sequencing (RNA-seq) method to sequence the whole transcriptome of the brain in small yellow croaker (). The animals were exposed to a mix of noises produced by different types of boat played back in a tank, then the brain tissues were collected after the fish had been exposed to a 120 dB noise for 0.5 h. In total, 762 differently expressed genes (DEGs) between the two groups were identified, including 157 up regulated and 605 down regulated genes in the noise exposure group compared with the control group. Gene ontology (GO) enrichment analysis indicated that the most up regulated gene categories included synaptic membranes, receptor-mediated endocytosis and the neurotransmitter secretion process. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways found that endocytosis, cell adhesion molecules and the extracellular matrix (ECM) receptor interaction pathway were over-represented. Specifically, ECM-related genes, including , , , , and were down regulated in the noise exposure group, implying the impaired composition of the ECM. In addition, the behavioral experiment revealed that exhibited avoidance behaviors to run away from the noise source at the beginning of the noise exposure period. At the end of the noise exposure period, kept motionless on the surface of the water and lost the ability to keep their balance. Taken together, our results indicate that exposure to noise stress contributes to neurological dysfunction in the brain and impaired locomotor ability in .
噪音有可能在海洋鱼类中诱发生理应激，这可能会导致各种生态后果。在当前的研究中，我们使用RNA测序（RNA-seq）方法对小黄鱼的大脑全转录组进行测序。将动物暴露于在水箱中播放的不同类型船只产生的混合噪音中，然后在鱼暴露于120分贝噪音0.5小时后收集脑组织。总共鉴定出两组之间762个差异表达基因（DEG），与对照组相比，噪音暴露组中有157个基因上调，605个基因下调。基因本体（GO）富集分析表明，上调最多的基因类别包括突触膜、受体介导的内吞作用和神经递质分泌过程。京都基因与基因组百科全书（KEGG）通路发现内吞作用、细胞粘附分子和细胞外基质（ECM）受体相互作用通路的占比过高。具体而言，与ECM相关的基因，包括 、 、 、 、 和 在噪音暴露组中下调，这意味着ECM的组成受损。此外，行为实验表明， 在噪音暴露期开始时表现出逃避行为以远离噪音源。在噪音暴露期结束时， 在水面上一动不动，失去了保持平衡的能力。综上所述，我们的结果表明，暴露于噪音应激会导致大脑神经功能障碍和 的运动能力受损。
