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超吸水性乳清蛋白/壳聚糖基抗菌气凝胶的制备、表征及其在鸡肉保鲜中的应用。

Superabsorbent whey protein isolates/chitosan-based antibacterial aerogels: Preparation, characterization and application in chicken meat preservation.

机构信息

College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.

College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.

出版信息

Int J Biol Macromol. 2024 Feb;259(Pt 1):128961. doi: 10.1016/j.ijbiomac.2023.128961. Epub 2023 Dec 26.

DOI:10.1016/j.ijbiomac.2023.128961
PMID:38154705
Abstract

Traditional absorbent pads are composed of hard-to-degrade polyethylene film and non-woven bottom layer, which have the characteristic of low absorption rate, without antibacterial effect. The objective of this study is to fabricate a novel superabsorbent and antibacterial aerogel, which consists of whey protein isolate (WPI) and chitosan (CS). The citric acid (CA) and ε-polylysine hydrochloride (ε-PLH) are incorporated into WPI/CS-based aerogel as cross-linking and antibacterial agent, respectively. The application in meat preservation as an absorbent pad is investigated. Results of water absorption, water vapor absorption and stress-strain show that aerogel comprised of 6 % WPI, 1.2 % CS, 2.0 % CA, and 2.0 % ε-PLH have the best water absorption capacity and stress. The density of WPI/CS/CA/ε-PLH aerogel is 82.7 ± 6.4 mg/cm, and has a uniform and polyporous microstructure, resulting in superabsorbent capacity. Antibacterial rate of WPI/CS/CA/ε-PLH aerogel against Staphylococcus aureus, Escherichia coli, Salmonella and Listeria monocytogenes reach around 80 %. The WPI/CS/CA/ε-PLH aerogel significantly reduces increased velocity of b, pH, total volatile base nitrogen, and total viable counts and decreased velocity of L and b of chicken meat (P < 0.05). Results indicate WPI/CS/CA/ε-PLH aerogel effectively extends shelf-life of chicken meat to 7 days, and could be used as an absorbent pad in meat preservation.

摘要

传统的吸收垫由难以降解的聚乙烯薄膜和无纺底层组成,其具有吸收率低、无抗菌作用的特点。本研究的目的是制备一种新型的超吸水性和抗菌气凝胶,由乳清蛋白分离物(WPI)和壳聚糖(CS)组成。柠檬酸(CA)和ε-聚赖氨酸盐酸盐(ε-PLH)分别作为交联剂和抗菌剂加入到 WPI/CS 基气凝胶中。将其作为吸收垫应用于肉类保鲜进行了研究。水吸收、水蒸气吸收和应力-应变结果表明,由 6% WPI、1.2% CS、2.0% CA 和 2.0% ε-PLH 组成的气凝胶具有最佳的水吸收能力和应力。WPI/CS/CA/ε-PLH 气凝胶的密度为 82.7±6.4mg/cm,具有均匀的多孔隙微观结构,从而具有超吸水性。WPI/CS/CA/ε-PLH 气凝胶对金黄色葡萄球菌、大肠杆菌、沙门氏菌和单核细胞增生李斯特菌的抑菌率均在 80%左右。WPI/CS/CA/ε-PLH 气凝胶显著降低了鸡肉 b 值、pH 值、总挥发性碱基氮和总活菌数的增加速度,降低了 L 值和 b 值(P<0.05)。结果表明,WPI/CS/CA/ε-PLH 气凝胶能有效延长鸡肉的货架期至 7 天,可作为肉类保鲜用吸收垫。

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