Han M, Chang M, Kim U J, Grunstein M
Cell. 1987 Feb 27;48(4):589-97. doi: 10.1016/0092-8674(87)90237-6.
To determine which cellular processes are dependent on histones, we blocked histone H2B mRNA synthesis in asynchronously growing yeast after fusing the H2B gene to a repressible GAL10 promoter. Chromosomal segregation, replication, and transcription were then examined. We found that the cells arrested in mitosis, with a cell division cycle (cdc) phenotype. Chromatin structure and nuclear segregation were disrupted. A full round of DNA replication took place after the repression of histone H2B mRNA synthesis. Active transcription and the induction of new transcripts also continued in the arrested cells.
为了确定哪些细胞过程依赖于组蛋白，我们将H2B基因与可抑制的GAL10启动子融合后，在异步生长的酵母中阻断组蛋白H2B mRNA的合成。然后检查染色体分离、复制和转录情况。我们发现细胞在有丝分裂中停滞，具有细胞分裂周期（cdc）表型。染色质结构和核分离被破坏。在组蛋白H2B mRNA合成受到抑制后，发生了一轮完整的DNA复制。在停滞的细胞中，活跃转录和新转录本的诱导也继续进行。
