Hereford L, Fahrner K, Woolford J, Rosbash M, Kaback D B
Cell. 1979 Dec;18(4):1261-71. doi: 10.1016/0092-8674(79)90237-x.
Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histones H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B.
对酵母组蛋白基因H2A和H2B的克隆序列分析表明，在单倍体酵母基因组中，这对基因只有两个拷贝。在每个拷贝中，H2A和H2B基因被大约700 bp的间隔DNA隔开。在酵母基因组中，这两个拷贝彼此之间的最小距离为35 - 60 kb。两个拷贝之间的序列同源性仅限于H2A和H2B基因；基因之间的间隔DNA是非同源的。在两个拷贝中，H2A和H2B基因都是反向转录的。此外，两个质粒都编码其他非组蛋白。在H2A和H2B基因的紧邻区域未检测到编码组蛋白H3和H4的序列。
