Fucci L, Vitale E, Cirotto C, Geraci G
Cell Differ. 1987 Jan;20(1):55-63. doi: 10.1016/0045-6039(87)90465-9.
Chemical identifications of various hemoglobin types were performed on unfractionated erythroid cells derived from chicken embryos at 5 and 7 days of development and on purified primitive and definitive cells. Proteins were pulse-labelled in primitive erythroid cells at various times of culture to identify those actually synthesized. The data show that primitive cells contain and synthesize only embryonic hemoglobins at all stages of maturation and definitive cells contain adult and minor embryonic hemoglobins, but no major embryonic hemoglobins, not even in trace amounts. These results support a model for hemoglobin switch in the chicken embryo based on cell line substitution.
对来自发育5天和7天鸡胚胎的未分级红细胞以及纯化的原始细胞和定型细胞进行了各种血红蛋白类型的化学鉴定。在培养的不同时间对原始红细胞中的蛋白质进行脉冲标记，以鉴定实际合成的蛋白质。数据表明，原始细胞在成熟的所有阶段都只含有并合成胚胎血红蛋白，而定型细胞含有成人血红蛋白和少量胚胎血红蛋白，但不含有主要胚胎血红蛋白，甚至连微量也没有。这些结果支持了基于细胞系替代的鸡胚胎血红蛋白转换模型。
