Du Zhichang, Chen Ling, Yang Shaohui
College of Marine Equipment and Mechanical Engineering, Jimei University, Xiamen, 361021, China.
Key Laboratory of Ocean Renewable Energy Equipment of Fujian Province, Xiamen, 361021, China.
Mikrochim Acta. 2023 Dec 29;191(1):65. doi: 10.1007/s00604-023-06140-z.
Microfluidic point-of-care testing (POCT) chips are used to enable the mixing and reaction of small sample volumes, facilitating target molecule detection. Traditional methods for actuating POCT chips rely on external pumps or power supplies, which are complex and non-portable. The development of finger-actuated chips has reduced operational difficulty and improved portability, promoting the development of POCT chips. This paper reviews the significance, developments, and potential applications of finger-actuated POCT chips. Three methods for controlling the flow accuracy of finger-actuated chips are summarized: direct push, indirect control, and sample injection control method, along with their respective advantages and disadvantages. Meanwhile, a comprehensive analysis of multi-fluid driving modes is provided, categorizing them into single-push multi-driving and multi-push multi-driving modes. Furthermore, recent research breakthroughs in finger-actuated chips are thoroughly summarized, and their structures, driving, and detection methods are discussed. Finally, this paper discusses the driving performance of finger-actuated chips, the suitability of detection scenarios, and the compatibility with existing detection technologies. It also provides prospects for the future development and application of finger-actuated POCT chips.
微流控即时检测（POCT）芯片用于实现小体积样品的混合和反应，便于目标分子检测。传统的驱动POCT芯片的方法依赖外部泵或电源，既复杂又不便于携带。手指驱动芯片的发展降低了操作难度，提高了便携性，推动了POCT芯片的发展。本文综述了手指驱动POCT芯片的意义、发展及潜在应用。总结了三种控制手指驱动芯片流动精度的方法：直接推动、间接控制和样品注入控制方法，以及它们各自的优缺点。同时，对多流体驱动模式进行了全面分析，将其分为单推多驱动和多推多驱动模式。此外，还全面总结了手指驱动芯片最近的研究突破，并讨论了其结构、驱动和检测方法。最后，本文讨论了手指驱动芯片的驱动性能、检测场景的适用性以及与现有检测技术的兼容性。还对手指驱动POCT芯片的未来发展和应用进行了展望。
