Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany.
Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam, Germany.
Metab Eng. 2024 Mar;82:12-28. doi: 10.1016/j.ymben.2023.12.012. Epub 2023 Dec 29.
Synthetic biology aims at designing new biological functions from first principles. These new designs allow to expand the natural solution space and overcome the limitations of naturally evolved systems. One example is synthetic CO-fixation pathways that promise to provide more efficient ways for the capture and conversion of CO than natural pathways, such as the Calvin Benson Bassham (CBB) cycle of photosynthesis. In this review, we provide a practical guideline for the design and realization of such new-to-nature CO-fixation pathways. We introduce the concept of "synthetic CO-fixation", and give a general overview over the enzymology and topology of synthetic pathways, before we derive general principles for their design from their eight naturally evolved analogs. We provide a comprehensive summary of synthetic carbon-assimilation pathways and derive a step-by-step, practical guide from the theoretical design to their practical implementation, before ending with an outlook on new developments in the field.
合成生物学旨在从根本上设计新的生物学功能。这些新设计允许扩展自然解决方案空间并克服自然进化系统的局限性。一个例子是合成 CO 固定途径，它有望提供比自然途径更有效的 CO 捕获和转化方式，例如光合作用的卡尔文-本森-巴斯汉姆（CBB）循环。在这篇综述中，我们为设计和实现这种新的自然 CO 固定途径提供了实用指南。我们介绍了“合成 CO 固定”的概念，并对合成途径的酶学和拓扑结构进行了概述，然后从它们的八个自然进化的类似物中推导出它们的设计的一般原则。我们对合成碳同化途径进行了全面总结，并从理论设计到实际实现，逐步提供了一个实用指南，最后展望了该领域的新发展。
