Laboratory of Organic Chemistry, ETH Zürich, 8093 Zürich, Switzerland.
Faraday Discuss. 2024 Sep 11;252(0):9-28. doi: 10.1039/d4fd00139g.
Enzymes are being engineered to catalyze chemical reactions for many practical applications in chemistry and biotechnology. The approaches used are surveyed in this short review, emphasizing methods for accessing reactivities not expressed by native protein scaffolds. The successful generation of completely enzymes that rival the rates and selectivities of their natural counterparts highlights the potential role that designer enzymes may play in the coming years in research, industry, and medicine. Some challenges that need to be addressed to realize this ambitious dream are considered together with possible solutions.
酶正在被设计用于催化化学反应，以应用于化学和生物技术的许多实际领域。本文综述了这些方法，强调了获取天然蛋白支架未表达的反应性的方法。完全人工酶的成功产生，其反应速率和选择性可与天然酶相媲美，这凸显了设计酶在未来几年的研究、工业和医学领域可能发挥的作用。本文还考虑了实现这一雄心勃勃的目标所需解决的一些挑战以及可能的解决方案。
