Shi Tianyu, Lure Maobulin, Zhang Run, Liu Zhiheng, Hu Qianming, Liu Jia, Yang Shengtao, Jing Liandong
Key Laboratory of Pollution Control Chemistry and Environmental Functional, Materials for Qinghai-Tibet Plateau of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu, 610041, China; Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu, 610041, China.
Key Laboratory of Pollution Control Chemistry and Environmental Functional, Materials for Qinghai-Tibet Plateau of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu, 610041, China; Key Laboratory of General Chemistry of the National Ethnic Affairs Commission, School of Chemistry and Environment, Southwest Minzu University, Chengdu, 610041, China.
Environ Res. 2024 Apr 1;246:118029. doi: 10.1016/j.envres.2023.118029. Epub 2023 Dec 30.
Livestock-polluted water is a pressing water environmental issue in plateau pastoral regions, necessitating the adoption of eco-friendly solutions. Despite periphyton being a promising alternative, its efficacy is limited by the prevalence of intense ultraviolet radiation, particularly ultraviolet-B (UVB), in these regions. Therefore, this study employs molecular tools and small-scale trials to explore the crucial role of indole-3-acetic acid (IAA) in modulating periphyton characteristics and mediating nutrient removal from livestock-polluted water under UVB exposure. The results revealed that IAA augments periphyton's resilience to UVB stress through several pathways, including increasing periphyton's biomass, producing more extracellular polymeric substances (EPS), and enhancing antioxidant enzyme activities and photosynthetic activity of periphyton. Moreover, IAA addition increased periphyton's bacterial diversity, reshaped bacterial community structure, enhanced community stability, and elevated the R value of neutral processes in bacterial assembly from 0.257 to 0.651 under UVB. Practically, an IAA concentration of 50 mg/L was recommended. Small-scale trials confirmed the effectiveness of IAA in assisting UVB-stressed periphyton to remove nitrogen and phosphorus from livestock-polluted water, without the risk of nitrogen accumulation. These findings offer valuable insights into the protection of aquatic ecosystems in plateau pastoral regions based on periphyton property in an eco-friendly manner.
牲畜污染水是高原牧区紧迫的水环境问题，需要采用环保解决方案。尽管附着生物是一种有前景的替代方案，但其功效受到这些地区强烈紫外线辐射、尤其是紫外线B（UVB）普遍存在的限制。因此，本研究采用分子工具和小规模试验，以探索吲哚-3-乙酸（IAA）在调节附着生物特性以及在UVB照射下介导从牲畜污染水中去除养分方面的关键作用。结果表明，IAA通过多种途径增强附着生物对UVB胁迫的恢复力，包括增加附着生物的生物量、产生更多胞外聚合物（EPS），以及增强附着生物的抗氧化酶活性和光合活性。此外，添加IAA增加了附着生物的细菌多样性，重塑了细菌群落结构，增强了群落稳定性，并使UVB照射下细菌组装中性过程的R值从0.257提高到0.651。实际上，建议IAA浓度为50 mg/L。小规模试验证实了IAA在协助受UVB胁迫的附着生物从牲畜污染水中去除氮和磷方面的有效性，且不存在氮积累风险。这些发现为以环保方式基于附着生物特性保护高原牧区水生生态系统提供了宝贵见解。
