Hausen B M, Kallweit M
Contact Dermatitis. 1986 Nov;15(5):289-94. doi: 10.1111/j.1600-0536.1986.tb01370.x.
5 coumarins used in perfumery, cosmetics, therapeutic ointments or occurring naturally were investigated by Freund's complete adjuvant technique (FCAT) in guinea pigs to determine their contact sensitizing potency. 4-Methylesculetin was also studied. Esculetin, dihydrocoumarin and daphnetin were found to be moderate to strong sensitizers, while fraxetin was nearly and 7-methylcoumarin completely inactive. The results corroborate the hypothetical view that only those coumarins having a catecholic disubstitution in the benzene ring, e.g., esculetin, 4-methylesculetin, daphnetin, can become sensitizers on the basis that they are capable of forming ortho-quinones under oxidizing conditions.
采用弗氏完全佐剂技术（FCAT）在豚鼠身上研究了用于香料、化妆品、治疗性软膏或天然存在的5种香豆素，以确定它们的接触致敏能力。还对4-甲基七叶亭进行了研究。发现七叶亭、二氢香豆素和瑞香素是中度至强致敏剂，而秦皮素几乎无活性，7-甲基香豆素完全无活性。结果证实了一种假设观点，即只有那些在苯环上具有邻苯二酚二取代的香豆素，如七叶亭、4-甲基七叶亭、瑞香素，由于它们在氧化条件下能够形成邻醌，所以能够成为致敏剂。
