Antidiabetic Effect of Standardized Hydroethanolic Extract by Targeting α-Glucosidase and the PTP-1B Signaling Pathway for Alleviating Diabetes in Experimental Model.

OBJECTIVES

The study's goal was to find out whether extract has anti-diabetic properties by concentrating on α-glucosidase and the PTP-1B signaling pathway. flowers were used for extraction using Methanol/water (80/20) as solvent.

METHODS

LC-MS techniques was used to check the presence of phytoconstituents present in extract. In vitro antidiabetic activity was evaluated using α-glucosidase inhibitory activity and PTP-1B signaling pathway. On Streptozotocin (STZ)-induced rats with diabetes, the in vivo antidiabetic efficacy was assessed using a test for oral glucose tolerance.

RESULTS

The phytoconstituents identified in the extract of were apigenin, diosmin, myricetin, luteolin, luteolin-7-glucoside, and Quercitrin as compound 1-6, respectively. Results showed that diosmin exhibited highest α-glucosidase inhibitory activity i.e. 90.39%. The protein level of PTP-1B was lowered and the insulin signalling activity was directly increased by compounds 1-6. The maximum blood glucose levels were seen in all groups' OGTT findings at 30 minutes following glucose delivery, followed by gradual drops. In comparison to the control group, the extract's glucose levels were 141 mg/dL at 30 minutes before falling to 104 mg/dL after 120 minutes. The current study has demonstrated, in summary, that extract with phytoconstituents reduce blood sugar levels in rats.

CONCLUSION

This finding suggests that extract may reduce the chance of insulin resistance and shield against disorders like hyperglycemia.