Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Korea.
Laboratories of Marine New Drugs, Redone Seoul, Seoul 08594, Korea.
Mar Drugs. 2021 Oct 30;19(11):618. doi: 10.3390/md19110618.
Four new chlorinated meroterpenoids, merochlorins G-J (-), and , a dihydronaphthalenedione precursor, along with known merochlorins A () and C-F (-), were obtained from cultivation of the bacterium strain sp. CNH-189, which was isolated from marine sediment. The planar structures of compounds - and were elucidated by interpretation of MS, UV, and NMR spectroscopic data. The relative configurations of compounds - were determined via analysis of nuclear Overhauser effect (NOE) spectroscopic data, after which their absolute configurations were established by comparing the experimental electronic circular dichroism (ECD) spectra of compounds - to those of previously reported possible enantiomer models and DP4 calculations. Compound displayed strong antibacterial activities against , , and , with MIC values of 1, 2, and 2 μg/mL, respectively, whereas compound exhibited weak antibacterial effects on these three strains, with a 16-32 μg/mL MIC value range.
从海洋沉积物中分离得到的菌株 sp. CNH-189 的发酵液中分离得到了 4 个新的氯化倍半萜类化合物 merochlorins G-J (-) 和 ，以及一个二氢萘二酮前体 ，以及已知的 merochlorins A () 和 C-F (-)。通过解析 MS、UV 和 NMR 光谱数据阐明了化合物 - 的平面结构。通过分析核奥弗豪瑟效应（NOE）光谱数据确定了化合物 - 的相对构型，然后通过将化合物 - 的实验电子圆二色性（ECD）光谱与先前报道的可能对映异构体模型和 DP4 计算进行比较，确定了它们的绝对构型。化合物 对 、 和 显示出较强的抗菌活性，MIC 值分别为 1、2 和 2 μg/mL，而化合物 对这三种菌株的抗菌活性较弱，MIC 值范围为 16-32 μg/mL。
