Boodhoo Nitish, Shojadoost Bahram, Alizadeh Mohammadali, Astill Jake, Behboudi Shahriar, Sharif Shayan
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Bristol Veterinary School, University of Bristol, Langford, Bristol, United Kingdom.
Front Microbiol. 2023 Dec 18;14:1257819. doi: 10.3389/fmicb.2023.1257819. eCollection 2023.
Alterations in intestinal microbiota can modulate the developing avian intestinal immune system and, subsequently, may impact on resistance to enteric pathogens. The aim was to demonstrate that early life exposure to , could affect either susceptibility or resistance of broilers to necrotic enteritis (NE). NZ9000 () pre-treatment at 1, 7, 14 and 21 days of age (DOA) led to a significant decrease in NE lesion scores in infected chickens. Infection was associated with spatial and temporal decreases in mononuclear phagocytes and CD4+ αβ T cells. However, pre-treatment and subsequent infection led to a significant increase in mononuclear phagocytes, CD8α + γδ T, αβ T cells (CD4+ and CD8α+) and B cells (IgM+, IgA+ and IgY+), as well as IL-12p40, IFN-γ and CD40. Differential expression of interleukin (IL)-6, IL-8, IL-10, IL-13, IL-18, IL-22, and transforming growth factor (TGF)-β were observed in treated chickens when compared to infected chickens. Microbiota analysis in infected chickens demonstrated an increase in abundance of Bacillota, Bacteroidota, Pseudomonadota and Actinomycetota. These findings suggests that modulation of the chicken intestinal immune system by confers partial protection 30 against NE.
肠道微生物群的改变可调节雏鸡肠道免疫系统的发育，进而可能影响对肠道病原体的抵抗力。目的是证明生命早期接触[具体物质未给出]会影响肉鸡对坏死性肠炎（NE）的易感性或抵抗力。在1、7、14和21日龄（DOA）进行NZ9000（[具体物质未给出]）预处理，可使感染鸡的NE病变评分显著降低。感染与单核吞噬细胞和CD4 + αβ T细胞的时空减少有关。然而，[具体物质未给出]预处理及随后的[具体物质未给出]感染导致单核吞噬细胞、CD8α + γδ T、αβ T细胞（CD4 + 和CD8α +）和B细胞（IgM +、IgA + 和IgY +）以及IL-12p40、IFN-γ和CD40显著增加。与[具体物质未给出]感染鸡相比，在[具体物质未给出]处理的鸡中观察到白细胞介素（IL）-6、IL-8、IL-10、IL-13、IL-18、IL-22和转化生长因子（TGF）-β的差异表达。对[具体物质未给出]感染鸡的微生物群分析表明，芽孢杆菌门、拟杆菌门、假单胞菌门和放线菌门的丰度增加。这些发现表明，[具体物质未给出]对鸡肠道免疫系统的调节赋予了对NE的部分保护作用。
