Abraham R, Charles A K, Mankes R, LeFevre R, Renak V, Ashok L
Ecotoxicol Environ Saf. 1986 Dec;12(3):213-9. doi: 10.1016/0147-6513(86)90013-8.
Preimplantation rat embryos (8-cell, morulae, blastocysts) were incubated for 24 hr in vitro in a Brinster medium with or without cadmium chloride (1 microgram/ml). CdCl2 arrested the development of both the 8-cell and morulae into blastocysts. Morphologic observations revealed the 8-cell embryo to have shrunken cells and pyknotic nuclei. Morulae appeared smaller in size with fewer cells than comparable controls. CdCl2 was clearly cytotoxic to the early blastocysts, inner mass cells were most damaged with cell death, pyknosis, and accumulation of debris.
将植入前的大鼠胚胎（8细胞期、桑葚胚、囊胚）在含有或不含有氯化镉（1微克/毫升）的布林斯特培养基中进行24小时体外培养。氯化镉阻止了8细胞期胚胎和桑葚胚发育为囊胚。形态学观察显示，8细胞期胚胎的细胞出现皱缩，细胞核固缩。与对照相比，桑葚胚体积更小，细胞数量更少。氯化镉对早期囊胚具有明显的细胞毒性，内细胞团细胞受损最为严重，出现细胞死亡、核固缩以及碎片堆积。
