Yu H S, Chan S T
Pharmacol Toxicol. 1987 Feb;60(2):129-34. doi: 10.1111/j.1600-0773.1987.tb01511.x.
Cadmium chloride, at concentrations of 0.5 or 1 microgram/ml medium, did not affect the trophoblastic invasiveness of mouse embryos treated for 24 hours at 4-cell and morula stages. At higher concentrations of 5 or 10 micrograms/ml medium, most treated embryos in vitro underwent degeneration while a few survivors formed trophoblastic outgrowths with variable areas. Cadmium chloride, at a low concentration of 0.5 microgram/ml medium presented continuously to blastocysts after attachment in vitro, has significantly retarded the trophoblastic outgrowth areas and reduced the number of trophoblastic giant-cell nuclei, though the spreading blastocysts appeared morphologically normal. At higher concentrations of 1 or 5 micrograms/ml medium, cytoplasmic disintegration and detachment of trophoblasts were observed. It is suggested that cadmium may interfere with the cell division and/or the transformation of trophectoderm cells into giant cells, resulting in the retardation of the trophoblastic outgrowths.
在培养基中浓度为0.5或1微克/毫升的氯化镉，对处于4细胞期和桑椹胚期处理24小时的小鼠胚胎的滋养层侵入性没有影响。在培养基中浓度较高（5或10微克/毫升）时，大多数体外处理的胚胎发生退化，而少数存活者形成了面积各异的滋养层生长物。在体外着床后持续给予囊胚低浓度（0.5微克/毫升培养基）的氯化镉，虽着床囊胚形态正常，但显著延缓了滋养层生长面积并减少了滋养层巨细胞核的数量。在较高浓度（1或5微克/毫升培养基）时，观察到滋养层细胞的细胞质解体和脱落。提示镉可能干扰细胞分裂和/或滋养外胚层细胞向巨细胞的转化，导致滋养层生长延缓。
