National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, P. R. China.
Qianxinan Agricultural Technology Extension Center, Xingyi 562404, P. R. China.
J Agric Food Chem. 2024 Jan 17;72(2):1007-1016. doi: 10.1021/acs.jafc.3c05659. Epub 2024 Jan 3.
RNA interference (RNAi) is a widespread post-transcriptional silencing mechanism that targets homologous mRNA sequences for specific degradation. An RNAi-based pest management strategy is target-specific and considered a sustainable biopesticide. However, the specific genes targeted and the efficiency of the delivery methods can vary widely across species. In this study, a spray-induced and nanocarrier-delivered gene silencing (SI-NDGS) system that incorporated gene-specific dsRNAs targeting conserved genes was used to evaluate phenotypic effects in white-backed planthopper (WBPH). At 2 days postspraying, transcript levels for all target genes were significantly reduced and knockdown of two gene orthologs, and resulted in an elevated mortality (>60%) and impaired ecdysis. These results highlight the utility of the SI-NDGS system for identifying genes involved in WBPH growth and development that could be potentially exploitable as high mortality target genes to develop an alternative method for WBPH control.
RNA 干扰 (RNAi) 是一种广泛存在的转录后沉默机制，可针对同源 mRNA 序列进行特异性降解。基于 RNAi 的害虫管理策略具有靶向特异性，被认为是一种可持续的生物农药。然而，特定的靶基因和递送方法的效率在不同物种之间可能有很大差异。在这项研究中，使用了一种喷雾诱导和纳米载体递送基因沉默 (SI-NDGS) 系统，该系统包含针对保守基因的基因特异性 dsRNA，以评估白背飞虱 (WBPH) 的表型效应。在喷雾后 2 天，所有靶基因的转录水平均显著降低，两个基因同源物 和 的敲低导致死亡率升高 (>60%) 和蜕皮受损。这些结果突出了 SI-NDGS 系统在鉴定参与 WBPH 生长和发育的基因方面的效用，这些基因可能作为高死亡率靶基因被利用，以开发替代的 WBPH 控制方法。
