Laboratory of Induced and Development Diseases, Medicine Faculty of Sfax University, Sfax, Tunisia.
Laboratory of Biomathematics LR22ES01, Faculty of Sciences of Sfax, Department of Mathematics, Sfax, Tunisia.
Toxicol Mech Methods. 2024 Jun;34(5):495-506. doi: 10.1080/15376516.2023.2301670. Epub 2024 Jan 26.
The current study aimed to assess the antioxidant and antiproliferative effects of extract: computational and study in rats. Three groups of animals: Group (i) constitute the control group; Group (ii) HeLa group received an intrafemoral inoculation of HeLa cells and Group (iii) constitue the combination between HeLa + . The plant was administered by gavage. Our results revealed that HeLa cell injection showed an elevation in aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), creatinine, urea, calcium and phosphorus. The pretreatment with the plant extract reduced the level of these parameters. Injection of HeLa cells showed a significant decrease in phosphorus and calcium respectively. However, the pretreatment by modulated the level of these two minerals. Rats treated with HeLa cells line showed an increase in the level of lipid peroxidation as evaluated by the TBARS substances, at the same time, a significant decreases in SOD, CAT and GPx activities were noted in the HeLa group compared to the control. On the other hand, pretreatment with the plant improved the level of these enzymes. Our results revealed that has a therapeutic effect on some health problems. HeLa cell line induced a small infiltration in liver and kidney. reduced the damage in both liver and kidney, but did not reveal any proliferation of tumor cells from trabecular bone tissue. The computational study revealed that compound bound with high free binding energies and established promising network of molecular interactions with COX-2 and TNF-α macromolecules.
本研究旨在评估 提取物的抗氧化和抗增殖作用：计算研究和大鼠体内研究。三组动物：第(i)组为对照组；第(ii)组为 HeLa 组，接受 HeLa 细胞股骨内接种；第(iii)组为 HeLa+ 组合组。植物通过灌胃给药。我们的结果表明，HeLa 细胞注射导致天冬氨酸转氨酶 (AST)、丙氨酸转氨酶 (ALT)、碱性磷酸酶 (ALP)、总胆红素 (TB)、肌酐、尿素、钙和磷升高。用植物提取物预处理降低了这些参数的水平。HeLa 细胞注射导致磷和钙水平分别显著降低。然而， 预处理调节了这两种矿物质的水平。用 HeLa 细胞系处理的大鼠表现出血清丙二醛 (TBARS)物质水平升高，脂质过氧化增加，同时 HeLa 组 SOD、CAT 和 GPx 活性与对照组相比显著降低。另一方面，用植物预处理提高了这些酶的水平。我们的结果表明， 对一些健康问题具有治疗作用。HeLa 细胞系在肝脏和肾脏中引起轻微浸润。 减少了肝脏和肾脏的损伤，但未显示出小梁骨组织中肿瘤细胞的任何增殖。计算研究表明， 化合物与高自由结合能结合，并与 COX-2 和 TNF-α 大分子建立了有前途的分子相互作用网络。
