Master of Biotechnology Program, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman, Jakarta, 12930, Indonesia.
Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University, Jalan Raya Dramaga, Bogor, 16680, Indonesia.
BMC Microbiol. 2020 Nov 12;20(1):343. doi: 10.1186/s12866-020-02022-z.
Indonesia is the third largest producer of fish and other aquaculture products in the world, making this industry a major contributor in the economy of Indonesia. However, this industry continually overcome challenges, one of them are bacterial outbreaks. In addition, the emergence of these bacterial outbreaks were worsen due to the biofilm produced by many significant pathogenic bacteria and the impact of increased antibiotic resistance. These issues have become a global concern, because antibiotics are currently one of the main treatments available to overcome this problems. Therefore, studies aimed at finding and characterizing bioactive compounds to combat these issues. In this study actinomycetes isolates were screened and characterized for their bioactive compounds produced which have inhibitory and destructive activity and also QS inhibitors against biofilm structure of aquatic pathogenic bacteria, such as Vibrio harveyi, A. hydrophila, and S. agalactiae.
Extracts (20 mg/mL) produced by sixteen Actinomycetes isolates showed anti-quorum sensing activity towards reporter stain Chromobacterium violaceum wild-type. Most of these extracts showed better inhibitory activity on all of the pathogenic bacteria biofilm structure tested than the destructive activity on the preformed of those biofilm structure. Subsequently, we also performed characterization of bioactive compound and found that in this study, polysaccharide is the most common antibiofilm agents, which were responsible to their antibiofilm activity. Finally, we found that the value of LC of all extracts tested were more than 1 mg/mL, thereby all of extracts tested did not show cyto-toxic effect against Artemia salina.
All of the extracts of Actinomycetes isolates showed promising inhibitory activity towards biofilm structure of pathogenic bacteria tested. So far, all of the extracts are potential to be QS inhibitors and antibiofilm agents of all pathogenic bacteria tested.
印度尼西亚是世界上第三大鱼类和其他水产养殖产品的生产国，这一产业是印度尼西亚经济的主要贡献者。然而，该行业不断面临挑战，其中之一是细菌爆发。此外，由于许多重要致病性细菌产生的生物膜以及抗生素耐药性增加的影响，这些细菌爆发的出现情况更加恶化。这些问题已经引起了全球的关注，因为抗生素是目前克服这些问题的主要治疗方法之一。因此，研究旨在寻找和表征具有抑制和破坏活性的生物活性化合物，以及针对水生致病性细菌生物膜结构的群体感应抑制剂，如哈维氏弧菌、嗜水气单胞菌和无乳链球菌。
从 16 株放线菌分离株中筛选出的提取物（20mg/ml）对报告菌株 Chromobacterium violaceum 野生型表现出抗群体感应活性。这些提取物中的大多数对所有测试的致病性细菌生物膜结构的抑制活性均优于对这些生物膜结构的预形成物的破坏活性。随后，我们还对生物活性化合物进行了表征，发现多糖是最常见的抗生物膜剂，其负责其抗生物膜活性。最后，我们发现所有测试提取物的 LC 值均大于 1mg/ml，因此所有测试提取物对卤虫均没有细胞毒性作用。
所有放线菌分离株的提取物均表现出对测试致病性细菌生物膜结构的有希望的抑制活性。到目前为止，所有提取物都有可能成为所有测试致病性细菌的群体感应抑制剂和抗生物膜剂。
