State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, People's Republic of China.
Appl Microbiol Biotechnol. 2024 Dec;108(1):16. doi: 10.1007/s00253-023-12892-8. Epub 2024 Jan 3.
Penicillium fungi, including Penicillium oxalicum, can secrete a range of efficient plant-polysaccharide-degrading enzymes (PPDEs) that is very useful for sustainable bioproduction, using renewable plant biomass as feedstock. However, the low efficiency and high cost of PPDE production seriously hamper the industrialization of processes based on PPDEs. In Penicillium, the expression of PPDE genes is strictly regulated by a complex regulatory system and molecular breeding to modify this system is a promising way to improve fungal PPDE yields. In this mini-review, we present an update on recent research progress concerning PPDE distribution and function, the regulatory mechanism of PPDE biosynthesis, and molecular breeding to produce PPDE-hyperproducing Penicillium strains. This review will facilitate future development of fungal PPDE production through metabolic engineering and synthetic biology, thereby promoting PPDE industrial biorefinery applications. KEY POINTS: • This mini review summarizes PPDE distribution and function in Penicillium. • It updates progress on the regulatory mechanism of PPDE biosynthesis in Penicillium. • It updates progress on breeding of PPDE-hyperproducing Penicillium strains.
青霉真菌，包括草酸青霉，可以分泌一系列高效的植物多糖降解酶（PPDEs），这对于使用可再生植物生物质作为原料的可持续生物生产非常有用。然而，PPDE 生产的低效率和高成本严重阻碍了基于 PPDE 的工艺的工业化。在青霉中，PPDE 基因的表达受到一个复杂的调控系统的严格调控，通过分子育种来修饰这个系统是提高真菌 PPDE 产量的一种有前途的方法。在这篇综述中，我们介绍了关于 PPDE 分布和功能、PPDE 生物合成调控机制以及产生 PPDE 高产青霉菌株的分子育种的最新研究进展。这篇综述将通过代谢工程和合成生物学促进真菌 PPDE 生产的未来发展，从而推动 PPDE 工业生物炼制应用。关键点：
本综述总结了青霉中 PPDE 的分布和功能。
更新了青霉中 PPDE 生物合成调控机制的研究进展。
更新了产生 PPDE 高产青霉菌株的育种进展。
