Gonzalez-Tobon Juliana, Helmann Tyler, Stodghill Paul, Filiatrault Melanie
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853.
United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853.
Phytopathology. 2024 May;114(5):1106-1117. doi: 10.1094/PHYTO-09-23-0351-KC. Epub 2024 Apr 22.
Bacteria belonging to the genus cause blackleg and soft rot symptoms on many plant hosts, including potato. Although there is considerable knowledge about the genetic determinants that allow to colonize host plants, as well as the genes that contribute to virulence, much is still unknown. To identify the genes important for fitness in potato stems, we constructed and evaluated randomly barcoded transposon mutant (RB-TnSeq) libraries of and . We identified 169 and 157 genes important for growth in and a in stems, respectively. This included genes related to metabolic pathways, chemotaxis and motility, transcriptional regulation, transport across membranes, membrane biogenesis, detoxification mechanisms, and virulence-related genes, including a potential virulence cluster , c-di-GMP modulating genes, and pectin degradation genes. When we compared the results of the stem assay with other datasets, we identified genes important for growth in stems versus tubers and in vitro conditions. Additionally, our data showed differences in fitness determinants for and . These data provide important insights into the mechanisms used by when interacting with and colonizing plants and thus might provide targets for management.
属于该属的细菌会在包括马铃薯在内的许多植物寄主上引发黑腿病和软腐病症状。尽管对于使该细菌能够定殖于寄主植物的遗传决定因素以及对毒力有贡献的基因已有相当多的了解，但仍有许多未知之处。为了鉴定在马铃薯茎中对适应性重要的基因，我们构建并评估了该细菌的随机条形码转座子突变体（RB-TnSeq）文库。我们分别鉴定出了169个和157个在茎中和a中对生长重要的基因。这包括与代谢途径、趋化性和运动性、转录调控、跨膜运输、膜生物合成、解毒机制以及毒力相关基因，包括一个潜在的毒力簇、环二鸟苷酸调节基因和果胶降解基因。当我们将茎试验的结果与其他数据集进行比较时，我们鉴定出了在茎与块茎以及体外条件下对生长重要的基因。此外，我们的数据显示了该细菌和a在适应性决定因素方面的差异。这些数据为该细菌与植物相互作用并定殖时所使用的机制提供了重要见解，因此可能为管理提供靶点。
