Maas D H, Stein B, Metzger H, Schneider U
Eur J Obstet Gynecol Reprod Biol. 1987 Jan;24(1):73-83. doi: 10.1016/0028-2243(87)90041-4.
The luminal pH and PO2 were measured in rabbit oviducts to evaluate the influence of microsurgery on the environment within the fallopian tube before and after resection of a 1 cm ampullary portion with subsequent tubal reanastomosis. While the oxygen tension showed no difference between the operated oviduct (56 +/- 16 mmHg, n = 8) and the control side (53 +/- 13 mmHg, n = 8), there was a significant pH decrease in the oviducts after microsurgery (7.94 +/- 0.10, n = 8, versus 7.75 +/- 0.16, n = 8, p less than 0.0025). In the second part of the experiment the operated animals were induced to ovulate by hCG injection, and artificial insemination was performed at the same time. The number of fertilized and unfertilized oocytes and the stages of embryonic development (64 h post hCG) were compared between the operated and control oviducts. From the operated oviducts a lower number of ova were recovered and a lower rate of fertilization was observed. The data indicate that microsurgery of the rabbit fallopian tube does not disturb the luminal oxygen tension but may cause a lower pH. Ultimately a reduced rate of fertilization and impaired early development were observed.
测量兔输卵管内的管腔pH值和氧分压，以评估在切除1 cm壶腹部部分并随后进行输卵管再吻合前后，显微手术对输卵管内环境的影响。虽然手术侧输卵管的氧分压（56±16 mmHg，n = 8）与对照侧（53±13 mmHg，n = 8）之间没有差异，但显微手术后输卵管内的pH值显著降低（7.94±0.10，n = 8，与7.75±0.16，n = 8相比，p<0.0025）。在实验的第二部分，通过注射hCG诱导手术动物排卵，并同时进行人工授精。比较手术侧和对照侧输卵管中受精卵和未受精卵的数量以及胚胎发育阶段（hCG注射后64小时）。从手术侧输卵管回收的卵子数量较少，受精率较低。数据表明，兔输卵管显微手术不会干扰管腔氧分压，但可能导致pH值降低。最终观察到受精率降低和早期发育受损。
