Maas D H, Stein B, Metzger H
Adv Exp Med Biol. 1984;169:561-70. doi: 10.1007/978-1-4684-1188-1_50.
Adhesions of the fallopian tube might be a source of generalized infertility. In order to improve the mechanical and functional properties of the oviduct, microsurgical replacement of the affected tubular segments have been applied, however, with contradictory results. In order to improve the situation and gain further information about this problem, catheter microelectrodes for Po2 and pH measurements within the lumen of the fallopian tube have been developed. The validity of the measurements was tested in a series of rabbits (Dutch belt strain, n = 19, 3.5-4.5 kg bw) which have been laparatomized under pentobarbital anesthesia during the oestrus phase. From one side of the oviduct 1 cm was resected and the free ends reanastomosed while the other side served as a control. Six to eight weeks later Po2 and pH values within the oviduct were measured. After another 4-5 weeks ovulation had been induced and artificial insemination performed. 64 hours later the rabbits were sacrificed. The oviducts were isolated and the corpora lutea as well as the ova were collected and examined histologically. The mean Po2 of the oviduct was 53 (+/- 16) mm Hg (mean +/- SD) for the control side and 56 (+/- 13) mm Hg (mean +/- SD) within the anastomosed tubes. The range of values was the same in both groups (n = 8) 34-75 mm Hg. A T-test comparison of the means of the two groups gave p-values which were not significant (p greater than 0.1). Po2 fluctuations with small-fast amplitudes (1 mm Hg) and additional slow-large oscillations (about 20 mm Hg) have been observed similar to the Po2 waves described for muscle and brain. The mean pH value of the oviduct a showed slight, statistically significant pH differences (7.75 reanastomosed side, 7.94 control side) whereby the degree of adhesions was correlated with luminal pH value. In three rabbits the relationship between corpora lutea and fertilized ova was 1:6, 2:9, 3:6 for the reanastomosed side while the control side showed balanced quotients of 4:4, 4:4, 6:6. In two rabbits unfertilized ova have been observed within the reanastomosed tubes where the typical outside layer of mucoproteids was absent while the control side was normal. Nevertheless, within both oviducts a continuous layer of epithelial cells has been observed even after reanastomosis.
输卵管粘连可能是导致全身性不孕的一个原因。为了改善输卵管的机械和功能特性，已采用显微外科手术替换受影响的管状节段，但结果却相互矛盾。为了改善这种情况并获取有关该问题的更多信息，已开发出用于测量输卵管腔内氧分压（Po2）和pH值的导管微电极。在一系列兔子（荷兰带纹品系，n = 19，体重3.5 - 4.5千克）身上对测量的有效性进行了测试，这些兔子在发情期接受戊巴比妥麻醉后进行了剖腹手术。从输卵管一侧切除1厘米，将游离端重新吻合，另一侧作为对照。6至8周后测量输卵管内的Po2和pH值。再过4 - 5周诱导排卵并进行人工授精。64小时后处死兔子。分离出输卵管，收集黄体和卵子并进行组织学检查。对照侧输卵管的平均Po2为53（±16）毫米汞柱（平均值±标准差），吻合管内为56（±13）毫米汞柱（平均值±标准差）。两组（n = 8）的值范围相同，为34 - 75毫米汞柱。对两组平均值进行T检验比较，得到的p值无统计学意义（p大于0.1）。观察到Po2有小而快的幅度波动（1毫米汞柱）以及额外的慢而大的振荡（约20毫米汞柱），类似于在肌肉和大脑中描述的Po2波。输卵管a的平均pH值显示出轻微的、具有统计学意义的pH差异（吻合侧为7.75，对照侧为7.94），粘连程度与管腔pH值相关。在三只兔子中，吻合侧黄体与受精卵的比例为1:6、2:9、3:6，而对照侧的比例为平衡的4:4、4:4、6:6。在两只兔子的吻合管内观察到未受精卵，那里缺少典型的粘蛋白外层，而对照侧正常。然而，即使在重新吻合后，两条输卵管内均观察到连续的上皮细胞层。
