IGeneX Inc. Milpitas; ID-FISH Technology Inc., California, USA.
IGeneX Inc. Milpitas, California, USA.
J Vector Borne Dis. 2023 Oct-Dec;60(4):353-364. doi: 10.4103/0972-9062.383641.
Lyme disease (LD) is caused by a group of tick-borne bacteria of the genus Borrelia termed Lyme disease Borreliae (LDB). The detection of serum antibodies to specific LDB antigens is widely used to support diagnosis of LD. Recent findings highlight a need for serological tests that can differentiate LD from tick-borne relapsing fever (TBRF) caused by a separate group of Borrelia species termed relapsing fever Borreliae. This is because LD and TBRF share some clinical symptoms and can occur in overlapping locations. The development of serological tests for TBRF is at an early stage compared with LD. This article reviews the application of line immunoblots (IBs), where recombinant proteins applied as lines on nitrocellulose membrane strips are used to detect antibodies in patient sera, for the diagnosis and differentiation of LD and TBRF.
莱姆病（LD）是由一组被称为莱姆病螺旋体（LDB）的蜱传细菌引起的。检测血清中针对特定 LDB 抗原的抗体广泛用于支持 LD 的诊断。最近的发现强调需要血清学检测方法，以区分由另一组被称为回归热螺旋体（relapsing fever Borreliae）的伯氏疏螺旋体引起的莱姆病和蜱传回归热（TBRF）。这是因为 LD 和 TBRF 具有一些共同的临床症状，并且可能发生在重叠的位置。与 LD 相比，TBRF 的血清学检测方法仍处于早期开发阶段。本文综述了线性免疫印迹（IBs）在 LD 和 TBRF 的诊断和鉴别中的应用，其中将重组蛋白作为线应用于硝酸纤维素膜条上，用于检测患者血清中的抗体。
