Grupo Biología de Sistemas, Escuela de Ciencias de la Salud, Facultad de Medicina, Universidad Pontificia Bolivariana, Circular 1a No 70-01, Bloque 11C - Oficina 417, Medellín, Colombia.
One Health, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
Comp Immunol Microbiol Infect Dis. 2024 Feb;105:102113. doi: 10.1016/j.cimid.2023.102113. Epub 2023 Dec 19.
Ticks are obligate ectoparasites and vectors of pathogens affecting health, agriculture, and animal welfare. This study collected ticks from the cattle and questing ticks of 24 Magdalena Medio Antioquia region cattle farms. Genomic DNA was extracted from the specimens (individual or pools) of the 2088 adult ticks collected from cattle and 4667 immature questing ticks collected from pastures. The molecular detection of Babesia, Anaplasma, Coxiella and Rickettsia genera was performed by polymerase chain reaction amplification and subsequent DNA sequencing. In a total of 6755 Rhipicephalus microplus DNA samples, Anaplasma marginale was the most detected with a frequency of 2% (Confidence Interval- CI 1.68-2.36), followed by Babesia bigemina with 0.28% (CI 0.16-0.44), Coxiella spp. with 0.15% (CI 0.07-0.27), and Rickettsia spp. with 0.13% (CI 0.06-0.25). Molecular analysis of the DNA sequences obtained from the tick samples revealed the presence of Coxiella-like endosymbiont and R. felis. These results demonstrated the diversity of microorganisms present in R. microplus ticks predominantly associated with cattle and questing ticks from livestock agroecosystems, suggesting their role as reservoirs and potential biological vectors of these microorganisms on the studied sites. Also, it emphasizes the need to combine acarological surveillance with clinical diagnoses and control strategies on regional and national levels.
蜱是专性外寄生虫，也是影响健康、农业和动物福利的病原体的载体。本研究从安蒂奥基亚省马格达莱纳中部地区的 24 个养牛场采集了牛蜱和游离蜱。从采集自牛的 2088 只成年蜱和采集自牧场的 4667 只未成熟游离蜱的标本（个体或混合）中提取基因组 DNA。通过聚合酶链反应扩增和随后的 DNA 测序，对巴贝虫、无形体、柯克斯体和立克次氏体属进行分子检测。在总共 6755 只 Rhipicephalus microplus DNA 样本中，检测到最多的是边缘无形体，频率为 2%（置信区间- CI 1.68-2.36），其次是双芽巴贝斯虫，频率为 0.28%（CI 0.16-0.44），柯克斯体，频率为 0.15%（CI 0.07-0.27），和立克次氏体，频率为 0.13%（CI 0.06-0.25）。从蜱样本中获得的 DNA 序列的分子分析显示存在柯克斯体样内共生体和 R. felis。这些结果表明，在 R. microplus 蜱中存在多种微生物，这些微生物主要与牛和牲畜农业生态系统中的游离蜱有关，表明它们作为这些微生物的储库和潜在生物载体的作用。此外，这强调了需要在区域和国家层面上结合节肢动物学监测与临床诊断和控制策略。
