Advanced Sensor and Energy Research (ASER) Laboratory, Department of Electronic Engineering, Kwangwoon University, 447-1, Seoul, 139-701, Republic of Korea.
Advanced Sensor and Energy Research (ASER) Laboratory, Department of Electronic Engineering, Kwangwoon University, 447-1, Seoul, 139-701, Republic of Korea.
Talanta. 2024 Apr 1;270:125582. doi: 10.1016/j.talanta.2023.125582. Epub 2023 Dec 21.
Despite substantial developments in minimally invasive lactate monitoring microneedle electrodes, most such electrode developments have focused on either sensitivity or invasiveness while ignoring a wide range of detection, which is the most important factor in measuring the normal range of lactate in interstitial fluid (ISF). Herein, we present a polymer-based planar microneedle electrode fabrication using microelectromechanical and femtosecond laser technology for the continuous monitoring of lactate in ISF. The microneedle is functionalized with two-dimensional reduced graphene oxide (rGO) and electrochemically synthesized platinum nanoparticles (PtNPs). A particular quantity of Nafion (1.25 wt%) is applied on top of the lactate enzyme to create a diffusion-controlled membrane. Due to the combined effects of the planar structure of the microneedle, rGO, and membrane, the biosensor exhibited excellent linearity up to 10 mM lactate with a limit of detection of 2.04 μM, high sensitivity of 43.96 μA mMcm, a reaction time of 8 s and outstanding stability, selectivity, and repeatability. The feasibility of the microneedle is evaluated by using it to measure lactate concentrations in artificial ISF and human serum. The results demonstrate that the microneedle described here has great potential for use in real-time lactate monitoring for use in sports medicine and treatment.
尽管微创乳酸监测微针电极取得了重大进展，但大多数此类电极的开发都集中在灵敏度或侵入性上，而忽略了检测范围广泛的问题，这是测量间质液（ISF）中乳酸正常范围的最重要因素。在此，我们提出了一种使用微机电和飞秒激光技术制造聚合物平面微针电极的方法，用于连续监测 ISF 中的乳酸。微针用二维还原氧化石墨烯（rGO）和电化学合成的铂纳米颗粒（PtNPs）功能化。在乳酸酶上施加一定量的 Nafion（1.25wt%），以形成扩散控制膜。由于微针的平面结构、rGO 和膜的综合作用，该生物传感器在高达 10mM 乳酸的范围内表现出优异的线性度，检测限为 2.04μM，灵敏度为 43.96μA·mM·cm，反应时间为 8s，具有出色的稳定性、选择性和可重复性。通过使用该微针测量人工 ISF 和人血清中的乳酸浓度来评估其可行性。结果表明，此处描述的微针在运动医学和治疗中的实时乳酸监测中具有很大的应用潜力。
