Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran.
Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran.
Biochimie. 2024 May;220:122-143. doi: 10.1016/j.biochi.2024.01.001. Epub 2024 Jan 3.
Miniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified into physical (label-free) and biological (labeled) methods based on their working principles. Each method has remarkable and feasible benefits for the purposes of cancer detection and therapy, as well as the challenges that we have discussed in this article. In this review, we present the recent advances in microfluidic cell sorting techniques that incorporate both physical and biological aspects, with an emphasis on the methods by which the cells are separated. We first introduce and discuss the biological cell sorting techniques, followed by the physical cell sorting techniques. Additionally, we explore the role of microfluidics in drug screening, drug delivery, and lab-on-chip (LOC) therapy. In addition, we discuss the challenges and future prospects of integrated microfluidics for cell sorting.
微型化在最近十年得到了显著改善，这使得许多微流控系统得以发展。微流控技术在从异质样品中分离所需细胞方面显示出巨大的潜力，因为它们具有样品消耗低、操作简单和分离精度高等优点。基于工作原理，微流控细胞分离方法可分为物理（无标记）和生物（标记）方法。每种方法对于癌症检测和治疗以及我们在本文中讨论的挑战都具有显著且可行的益处。在这篇综述中，我们介绍了结合物理和生物方面的微流控细胞分选技术的最新进展，重点介绍了细胞分离的方法。我们首先介绍和讨论生物细胞分选技术，然后介绍物理细胞分选技术。此外，我们还探讨了微流控在药物筛选、药物输送和芯片实验室（LOC）治疗中的作用。此外，我们还讨论了集成微流控用于细胞分选的挑战和未来前景。
