Mohammed Dina Mostafa, El-Messery Tamer M, Baranenko Denis A, Hashim Mahmood A, Boulkrane Mohamed Said, El-Said Marwa M
Nutrition and Food Sciences Department, National Research Centre, Cairo, Egypt.
International Research Centre "Biotechnologies of the Third Millennium", Faculty of Biotechnologies (BioTech), ITMO University, St. Petersburg, Russia.
Front Nutr. 2023 Dec 18;10:1273299. doi: 10.3389/fnut.2023.1273299. eCollection 2023.
The consumption of dairy products, including soft cheese, has been associated with numerous health benefits due to their high nutritional value. However, the phenolic compounds bioaccessibility present in soft cheese is limited due to their poor solubility and stability during digestion. So, this study aimed to develop an innovative soft cheese enriched with date seed phenolic compounds (DSP) extracted ultrasonically and incorporated into homogeneous liposomes and study its attenuation effect on testosterone-induced benign prostatic hyperplasia (BPH) in rats.
Date seed phenolic compounds were extracted using 98 and 50% ethanol along with water as solvents, employing ultrasonication at 10, 20, and 30-min intervals. The primary and secondary DSP-liposomes were prepared and dehydrated. The particle size, zeta potential, encapsulation efficiency, and morphology were measured. Incorporating dehydrated liposomes (1-3% w/w) into soft cheese and their impact on BPH using male Sprague-Dawley rats was assessed. After inducing BPH, rats were fed a cheese diet with dehydrated DSP-liposomes. Over 8 weeks, parameters including nutrition parameters, prostate enlargement analysis, biochemical parameters, hormones level, oxidative stress, and cytokines were analyzed.
The results showed that ultrasound-assisted extraction effectively reduced the extraction time and 30 min extraction EtOH 50% was enough to extract high yield of phenolic compounds (558 mg GA/g) and flavonoids (55 mg qu/g) with high antioxidant activity (74%). The biological results indicate that prostate weight and prostate index% were diminished in the treatment groups (1 and 2) compared to the BPH control group. The high antioxidant content present in the DSP-liposomes acted as the catalyst for suppressing the responses of the inflammatory cytokines, inhibiting the anti-inflammatory IL-10 production, and suppressing the elevated levels of lipid peroxidation products compared to the BPH group.
The treatment group (2) supplemented with dehydrated secondary DSP-liposomes exhibited the most significant variance ( < 0.05) as opposed to the BPH group. Liposomal encapsulation was proved to be a feasible approach for administering DSP in soft cheese, thereby establishing new functional food category possessing prophylactic properties against the advancement of BPH in rats.
由于乳制品具有较高的营养价值，食用乳制品（包括软奶酪）对健康有诸多益处。然而，软奶酪中酚类化合物的生物可及性有限，因为它们在消化过程中的溶解性和稳定性较差。因此，本研究旨在开发一种创新的软奶酪，其中富含通过超声提取并包裹在均匀脂质体中的枣籽酚类化合物（DSP），并研究其对大鼠睾酮诱导的良性前列腺增生（BPH）的缓解作用。
以98%和50%乙醇以及水为溶剂，采用超声处理，间隔10、20和30分钟提取枣籽酚类化合物。制备并脱水初级和次级DSP脂质体。测量其粒径、zeta电位、包封率和形态。将脱水脂质体（1-3% w/w）加入软奶酪中，并评估其对雄性Sprague-Dawley大鼠BPH的影响。诱导BPH后，给大鼠喂食含有脱水DSP脂质体的奶酪饮食。在8周内，分析包括营养参数、前列腺肿大分析、生化参数、激素水平、氧化应激和细胞因子等参数。
结果表明，超声辅助提取有效缩短了提取时间，30分钟的50%乙醇提取足以提取高产率的酚类化合物（558毫克GA/克）和黄酮类化合物（55毫克qu/克），且具有较高的抗氧化活性（74%）。生物学结果表明，与BPH对照组相比，治疗组（1和2）的前列腺重量和前列腺指数%有所降低。与BPH组相比，DSP脂质体中高含量的抗氧化剂起到了抑制炎性细胞因子反应、抑制抗炎性IL-10产生以及抑制脂质过氧化产物水平升高的催化剂作用。
与BPH组相比，添加脱水次级DSP脂质体的治疗组（2）表现出最显著的差异（<0.05）。脂质体包封被证明是在软奶酪中施用DSP 的可行方法，从而建立了一种对大鼠BPH进展具有预防特性的新型功能性食品类别。
