Rodrigues Vinícius Bley, Menezes Júlia Machado, da Silva Laura Cé, Müller Isadora, Mallmann Larissa, Hermann Bruna Saraiva, Menezes Charlene, Brucker Natália, da Vida Roselaine L, Picada Jaqueline Nascimento, Boaretto Fernanda Brião Menezes, Schneider Anelise, Linden Rafael, Zanella Renato, Fleck Juliane Deise, Charão Mariele Feiffer
Laboratory of Analytical Toxicology, Post-graduation in Toxicology and Toxicological Analysis, Feevale University, ERS 239, 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil.
Laboratory of Molecular Microbiology, Post-graduation in Virology, Feevale University, ERS 239, 2755, Novo Hamburgo, RS 93352-000, Brazil.
Toxicol Res (Camb). 2023 Dec 18;13(1):tfad117. doi: 10.1093/toxres/tfad117. eCollection 2024 Feb.
Urbanization and agricultural activities increased environmental contaminants. Integrated analysis of water parameters and bioassays represents an essential approach to evaluating aquatic resource quality. This study aimed to assess water quality by microbiological and physicochemical parameters as well as the toxicological effects of water samples on the Ames test and model. Samples were collected during (collection 1) and after (collection 2) pesticide application in the upper (S1), middle (S2), and lower (S3) sections of the Rolante River, southern Brazil. Metals were determined by GFAAS and pesticides by UPLC-MS/MS. Bioassays using the Ames test and the nematode Levels of microbiological parameters, as well as Mn and Cu were higher than the maximum allowed limits established by legislation in collection 2 compared to collection 1. The presence of pesticide was observed in both collections; higher levels were found in collection 1. No mutagenic effect was detected. Significant inhibition of body length of was found in collection 1 at S2 ( < 0.001) and S3 ( < 0.001) and in collection 2 at S2 ( = 0.004). Comparing the same sampling site between collections, a significant difference was found between the site of collection (F(3,6)=8.75, = 0.01) and the time of collection (F(1,2)=28.61, = 0.03), for the S2 and S3 samples. model was useful for assessing surface water quality/toxicity. Results suggest that an integrated analysis for the surface water status could be beneficial for future approaches.
城市化和农业活动增加了环境污染物。对水参数和生物测定进行综合分析是评估水生资源质量的重要方法。本研究旨在通过微生物和理化参数评估水质，以及水样对艾姆斯试验和模型的毒理学影响。在巴西南部罗兰特河上游（S1）、中游（S2）和下游（S3）的农药施用期间（采集1）和之后（采集2）采集样本。通过石墨炉原子吸收光谱法（GFAAS）测定金属，通过超高效液相色谱-串联质谱法（UPLC-MS/MS）测定农药。使用艾姆斯试验和线虫进行生物测定。与采集1相比，采集2中微生物参数以及锰和铜的水平高于法规规定的最大允许限值。在两次采集中均观察到农药的存在；在采集1中发现的含量更高。未检测到致突变作用。在采集1的S2（P<0.001）和S3（P<0.001）以及采集2的S2（P=0.004）中发现线虫体长受到显著抑制。比较两次采集之间的相同采样点，对于S2和S3样本，在采集地点（F(3,6)=8.75，P=0.01）和采集时间（F(1,2)=28.61，P=0.03）之间发现了显著差异。该模型对于评估地表水质量/毒性很有用。结果表明，对地表水状况进行综合分析可能对未来的方法有益。
