College of Acupuncture and Tuina, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
Department of Tuina, Shandong University of Traditional Chinese Medicine Affiliated Hospital, Jinan, Shandong, China.
Sci Rep. 2024 Jan 5;14(1):661. doi: 10.1038/s41598-023-50904-9.
Frozen shoulder (FS) is a common disorder often treated with Tuina, but the mechanisms involved remain unclear. We employed proteomics and phosphoproteomics to investigate the mechanisms associated with the treatment of capsule fibrosis in FS rats. We used a method composed of three weeks of cast immobilization to establish a model of FS. We then administered Tuina once daily for 14 days, evaluated glenohumeral range of motion (ROM), assessed histological changes, and identified differentially expressed proteins (DEPs) using proteomics and phosphoproteomics. This study demonstrated that Tuina could improve glenohumeral ROM and reserve capsule fibrosis in FS rats. Proteomics revealed proteins regulated by Tuina belonging to the PI3K-AKT and ECM receptor interaction signaling pathways. Phosphoproteomics detected differentially phosphorylated proteins regulated by Tuina to be enriched in the MAPK signaling pathway. The combination of proteomics and phosphoproteomics for Protein-Protein Interaction (PPI) network analysis revealed that the phosphorylation of Myh3 and Srsf1 with a node degree larger than the average degree were considered the central regulatory protein modulated by Tuina to reverse capsule fibrosis. Thbs1, Vtn, and Tenascin-W were significantly enriched in PI3K-AKT and ECM receptor interaction signaling pathways and highly expressed in model rats. Tuina resulted in reduced expression of these proteins. Our findings demonstrated some of mechanisms behind the reversal of FS capsule fibrosis following Tuina, a scientific medical therapy for FS patients.
冻结肩（FS）是一种常见疾病，常采用推拿疗法进行治疗，但相关机制尚不清楚。我们采用蛋白质组学和磷酸化蛋白质组学方法，研究推拿治疗 FS 大鼠囊纤维化的相关机制。我们采用三周的石膏固定法建立 FS 模型。然后，我们每天进行一次推拿治疗，共 14 天，评估盂肱关节活动度（ROM），评估组织学变化，并采用蛋白质组学和磷酸化蛋白质组学方法鉴定差异表达蛋白（DEPs）。本研究表明，推拿可以改善 FS 大鼠盂肱关节 ROM 和储备囊纤维化。蛋白质组学显示，推拿调节的 PI3K-AKT 和 ECM 受体相互作用信号通路中的蛋白。磷酸化蛋白质组学检测到推拿调节的差异磷酸化蛋白富集在 MAPK 信号通路中。蛋白质组学和磷酸化蛋白质组学的蛋白-蛋白相互作用（PPI）网络分析显示，Myh3 和 Srsf1 的磷酸化程度大于平均程度，被认为是推拿调节逆转囊纤维化的核心调节蛋白。Thbs1、Vtn 和 Tenascin-W 在 PI3K-AKT 和 ECM 受体相互作用信号通路中显著富集，在模型大鼠中高表达。推拿导致这些蛋白的表达减少。我们的研究结果表明，推拿治疗 FS 患者的 FS 囊纤维化逆转的部分机制。
