一种用67Ga标记的抗体-去铁胺偶联物。

An antibody-desferrioxamine conjugate labelled with 67Ga.

作者信息

Ward M C, Roberts K R, Babich J W, Bukhari M A, Coghlan G, Westwood J H, McCready V R, Ott R J

出版信息

Int J Rad Appl Instrum B. 1986;13(5):505-7. doi: 10.1016/0883-2897(86)90128-5.

Abstract

The optimum conditions for producing a 67Ga labelled antibody-desferrioxamine conjugate were determined. The mouse monoclonal antibody LICR-LON-M8 was coupled to the metal chelating compound desferrioxamine (DFO) using glutaraldehyde (GLUT). A DFO:GLUT:M8 molar ratio of 500:150:1 gave an immunoreactive antibody with low amounts (less than 5%) of high molecular weight polymer. The labelling efficiency with 67 Ga was greater than 90%, with a specific activity of 2-4 MBq/mg antibody. This 67Ga labelled antibody is suitable for evaluation as a diagnostic imaging agent.

摘要

确定了生产67Ga标记的抗体-去铁胺偶联物的最佳条件。使用戊二醛（GLUT）将小鼠单克隆抗体LICR-LON-M8与金属螯合化合物去铁胺（DFO）偶联。DFO:GLUT:M8的摩尔比为500:150:1时，可得到具有少量（小于5%）高分子量聚合物的免疫反应性抗体。67Ga的标记效率大于90%，比活度为2-4 MBq/mg抗体。这种67Ga标记的抗体适合作为诊断成像剂进行评估。