Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Qareeb St., Azadi Ave, Tehran, Iran.
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
BMC Vet Res. 2024 Jan 6;20(1):14. doi: 10.1186/s12917-023-03862-3.
The Zarudny's spur-thighed tortoise or Iranian tortoise (Testudo graeca zarudnyi) has just been reported from Iran so far. Therefore, the purpose of this study was to determine the effects of season and gender on serum biochemical parameters of this valuable species in Iran.
This study was performed on 20 clinically healthy adult Zarudny's spur-thighed tortoises. Blood samples were collected from the jugular vein and then serum biochemical parameters and body weight were measured in autumn and winter. The following biochemical parameters were measured: total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, very low-density lipoprotein, total protein, creatinine, urea, glucose, calcium, inorganic phosphorus, total bilirubin, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase. Urea concentration increased significantly at emergence from hibernation. Also, in winter, total protein, phosphorous, creatinine, total bilirubin concentrations and alkaline phosphatase activity decreased significantly compared to autumn, but aspartate aminotransferase and lactate dehydrogenase activities were significantly higher than in autumn. There was no significant difference for the parameters mentioned above by gender, except for phosphorous. Phosphorous concentration in females was significantly higher than that of males in autumn. Cholesterol and low-density lipoprotein cholesterol levels in females were significantly higher than males in autumn and winter.
The present study is the first study that monitors the serum biochemical parameters of adult Zarudny's spur-thighed tortoises based on season and gender. Seasonal reference intervals should be used for biochemical parameters in this valuable species. Also, sex-specific reference intervals for phosphate and cholesterol are necessary.
迄今为止，扎鲁丁腿刺龟或伊朗龟（Testudo graeca zarudnyi）仅在伊朗有报道。因此，本研究的目的是确定季节和性别对该珍稀物种血清生化参数的影响。
本研究对 20 只临床健康的成年扎鲁丁腿刺龟进行了研究。从颈静脉采集血液样本，然后在秋季和冬季测量血清生化参数和体重。测量了以下生化参数：总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、极低密度脂蛋白、总蛋白、肌酐、尿素、葡萄糖、钙、无机磷、总胆红素、尿酸、丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶和乳酸脱氢酶。冬眠后苏醒时尿素浓度显著升高。此外，与秋季相比，冬季总蛋白、磷、肌酐、总胆红素浓度和碱性磷酸酶活性显著降低，但天冬氨酸氨基转移酶和乳酸脱氢酶活性显著高于秋季。除磷外，上述参数在性别之间无显著差异。秋季雌性磷浓度显著高于雄性。胆固醇和低密度脂蛋白胆固醇水平在秋季和冬季雌性显著高于雄性。
本研究首次监测了成年扎鲁丁腿刺龟基于季节和性别的血清生化参数。在该珍稀物种中，应使用季节性参考区间进行生化参数检测。此外，还需要针对磷酸盐和胆固醇制定性别特异性参考区间。
