利用亚致死毒性损伤的敏感指标对心血管毒素进行的比较研究。

The comparative study of cardiovascular toxins utilizing a sensitive indicator of sublethal toxic injury.

作者信息

Hysmith R H, Welch T K, Boor P J

出版信息

In Vitro Cell Dev Biol. 1987 Feb;23(2):129-33. doi: 10.1007/BF02623593.

Abstract

This study measures the inhibition of [3H]uridine uptake by smooth muscle cells to determine sublethal toxic injury by the cardiovascular toxins, allylamine, isoproterenol, and beta-aminopropionitrile. The exposure period or the concentration of toxin which inhibited 30% of [3H]uridine uptake by smooth muscle cells could be utilized as an endpoint for ranking toxicity. Cytotoxicity of the three toxins to smooth muscle cells were ranked as: allylamine greater than isoproterenol greater than beta-aminopropionitrile. Recovery of cells utilizing [3H]uridine uptake inhibition as a method for assessing comparative cytotoxicity and for screening of agents potentially injurious to vascular cells.

摘要

本研究通过测量平滑肌细胞对[3H]尿苷摄取的抑制作用，来确定心血管毒素烯丙胺、异丙肾上腺素和β-氨基丙腈所致的亚致死性毒性损伤。平滑肌细胞对[3H]尿苷摄取抑制30%时的暴露时间或毒素浓度，可作为毒性分级的终点指标。三种毒素对平滑肌细胞的细胞毒性分级为：烯丙胺＞异丙肾上腺素＞β-氨基丙腈。利用[3H]尿苷摄取抑制来评估细胞毒性及筛选可能对血管细胞有损伤作用的药物，以实现细胞的恢复。