Rajakylä E, Laasasenaho K, Sakkers P J
J Chromatogr. 1987 Jan 2;384:391-402. doi: 10.1016/s0021-9673(01)94686-2.
An high-performance liquid chromatographic method is described for determination of deoxynivalenol, patulin, diacetoxyscirpenol, HT-2-toxin, T-2-toxin, zearalenone and ochratoxin A using a reversed-phase column and a diode-array detector. The extraction and purification steps and optimum chromatographic conditions are described. Detection limits and recoveries from spiked wheat samples were investigated. The combination of the high-performance liquid chromatographic system described together with a modern thermospray quadrupole mass spectrometer is a very specific and sensitive method for analyzing a wide range of mycotoxins in biological samples.
描述了一种高效液相色谱法，用于使用反相柱和二极管阵列检测器测定脱氧雪腐镰刀菌烯醇、展青霉素、二乙酰氧基雪腐镰刀菌烯醇、HT-2毒素、T-2毒素、玉米赤霉烯酮和赭曲霉毒素A。描述了提取和纯化步骤以及最佳色谱条件。研究了加标小麦样品的检测限和回收率。所描述的高效液相色谱系统与现代热喷雾四极杆质谱仪相结合，是一种分析生物样品中多种霉菌毒素的非常特异且灵敏的方法。
